Rabbit Anti-IRS-1 Polyclonal Antibody – Cat# MBS302159

Human colon carcinoma stained with anti-IRS-1 antibody

IRS-1 Antibody – Rabbit Polyclonal

Name: IRS-1 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS302159
Price: 390 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS302159

The insulin receptor substrate-1 (IRS-1), a 170-185kDa protein major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor (IGF-I) and interleukin 4. IRS-1 is phosphorylated on serine,… More details

$390 / 0.5 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IRS-1, Polyclonal Antibody

Full Product Name

Rabbit Anti-Human IRS-1 Polyclonal Antibody

Product Gene Name

anti-IRS-1 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human
Predicted Reactivity: Chicken, Dog, Mouse, Pig, Rabbit, Rat

Form/Format

0.5 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Preparation and Storage

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Molecular Weight

170-185 kDa

OMIM

125853

Immunogen

Synthetic peptide corresponding to aa 1223-1242 of human IRS-1.

Control Tissue

Colon Carcinoma

Cellular Localization

Cytoplasm

Entrez Gene Code

3667

Pathway

Signal Transduction

Applications Tested/Suitable for anti-IRS-1 antibody

Immunohistochemistry (IHC) Paraffin

Application Notes for anti-IRS-1 antibody

Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:50
IHC Positive Control: Colon Carcinoma

Other Notes

Small volumes of anti-IRS-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IRS-1 purchase

MBS302159 is an antibody targeting IRS-1. Also known as Rabbit Anti-Human IRS-1 Polyclonal Antibody. Reactive in Human
Predicted Reactivity: Chicken, Dog, Mouse, Pig, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IRS-1. It may not necessarily be applicable to this product.

NCBI GI #

5031805

NCBI GeneID

3667

NCBI Accession #

NP_005535.1

UniProt Related Accession #

P35568

GenBank Nucleotide #

NM_005544.2

NCBI Official Full Name

insulin receptor substrate 1

NCBI Official Synonym Full Names

insulin receptor substrate 1

NCBI Official Symbol

IRS1

NCBI Official Synonym Symbols

HIRS-1

UniProt Protein Name

Insulin receptor substrate 1

Protein Family

PH-interacting protein

UniProt Gene Name

IRS1

UniProt Synonym Gene Names

IRS-1

UniProt Entry Name

IRS1_HUMAN

NCBI Protein Information

insulin receptor substrate 1; IRS-1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IRS-1 antibody

Reference Product	PubMed Publications
IGF1R antibody	>246 publications with IRS-1 and IGF1R
GRB2 antibody	>138 publications with IRS-1 and GRB2
JAK2 antibody	>63 publications with IRS-1 and JAK2
INSR antibody	>58 publications with IRS-1 and INSR
PTPN11 antibody	>42 publications with IRS-1 and PTPN11
JAK1 antibody	>26 publications with IRS-1 and JAK1
MAPK8 antibody	>23 publications with IRS-1 and MAPK8
PIK3R1 antibody	>12 publications with IRS-1 and PIK3R1
PIK3CA antibody	>6 publications with IRS-1 and PIK3CA

Pathways associated with anti-IRS-1 antibody

Products by Pathway	Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
AMPK Signaling Pathway antibodies	AMPK Signaling Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Aldosterone-regulated Sodium Reabsorption Pathway antibodies	Aldosterone-regulated Sodium Reabsorption Pathway Diagram
Aldosterone-regulated Sodium Reabsorption Pathway antibodies	Aldosterone-regulated Sodium Reabsorption Pathway Diagram
Alpha6-Beta4 Integrin Signaling Pathway antibodies	Alpha6-Beta4 Integrin Signaling Pathway Diagram

Diseases associated with anti-IRS-1 antibody

Disease Name	Pubmed Publications
Insulin Resistance Antibodies	>1262 publications with IRS-1 and Insulin Resistance
Hyperinsulinism Antibodies	>860 publications with IRS-1 and Hyperinsulinism
Diabetes Mellitus Antibodies	>508 publications with IRS-1 and Diabetes Mellitus
Obesity Antibodies	>428 publications with IRS-1 and Obesity
Diabetes Mellitus, Type 2 Antibodies	>347 publications with IRS-1 and Diabetes Mellitus, Type 2
Disease Models, Animal Antibodies	>206 publications with IRS-1 and Disease Models, Animal
Hyperglycemia Antibodies	>186 publications with IRS-1 and Hyperglycemia
Necrosis Antibodies	>177 publications with IRS-1 and Necrosis
Inflammation Antibodies	>138 publications with IRS-1 and Inflammation
Liver Diseases Antibodies	>136 publications with IRS-1 and Liver Diseases

Organs/Tissues associated with anti-IRS-1 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>874 publications with IRS-1 and Muscle
Liver Antibodies	>493 publications with IRS-1 and Liver
Connective Tissue Antibodies	>250 publications with IRS-1 and Connective Tissue
Pancreas Antibodies	>146 publications with IRS-1 and Pancreas
Heart Antibodies	>138 publications with IRS-1 and Heart
Brain Antibodies	>126 publications with IRS-1 and Brain
Ovary Antibodies	>113 publications with IRS-1 and Ovary
Kidney Antibodies	>97 publications with IRS-1 and Kidney
Nerve Antibodies	>46 publications with IRS-1 and Nerve
Bone Antibodies	>46 publications with IRS-1 and Bone