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IFN Alpha (IFNalpha) Antibody – Hamster Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3030145

MBS3030145 is an antibody targeting IFNalpha. Also known as Anti-Mouse IFN alpha In Vivo Antibody - Ultra Low Endotoxin (TIF-3C5). Reactive in Mouse. Host: Armenian Hamster. More details

$675 / 5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IFN alpha (IFNalpha), Monoclonal Antibody
Full Product Name
Anti-Mouse IFN alpha In Vivo Antibody - Ultra Low Endotoxin (TIF-3C5)
Product Gene Name
anti-IFNalpha antibody
Product Synonym Names
IFNa; interferon alpha
Clonality
Monoclonal
Isotype
Armenian Hamster IgG
Clone Number
TIF-3C5
Host
Armenian Hamster
Species Reactivity
Mouse
Form/Format
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purity/Purification
>98% by SDS-PAGE and HPLC
Concentration
>=2.0mg/ml (lot specific)
Specificity
Anti-IFN alpha In Vivo Antibody - Low Endotoxin (TIF-3C5) recognizes an epitope on Mouse IFNalpha and binds specifically to murine IFNa species (IFN-alpha-A, -1, -4, -5, -11. and -13) and does not bind murine murine IFNg or IFNb
Preparation and Storage
anti-IFN alpha In Vivo Antibody - Ultra Low Endotoxin (TIF-3C5) is stable when stored at 2-8 degree C. For long-term storage aseptically aliquot in working volumes without diluting and store at -80 degree C. Avoid Repeated Freeze Thaw Cycles.
Matching Pairs
Antibody: IFNalpha Clone #TIF-3C5 (MBS3030145)
Isotype Control: GST Clone #PIP (MBS3030272)
Molecular Weight
21,646 Da
3D Structure
ModBase 3D Structure for P01572
GMP
Manufactured according to cGMP (Current Good Manufacturing Practice) standards.
Endotoxin
<= 0.75 EU/mg as determined by the LAL method
Aggregation
Aggregation level <= 1%
IMPACT Pathogen Test
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Hamster parvovirus Lymphocytic choriomeningitis Minute virus of mice Theiler's murine encephalomyelitis virus Sendai virus Reovirus 3 Hantaan virus

Product Categories/Family for anti-IFNalpha antibody

Ultra Low Endotoxin; anti-mouse
Applications Tested/Suitable for anti-IFNalpha antibody
Western Blot (WB), Functional Assay (FUNC)
Application Notes for anti-IFNalpha antibody
FUNC: In vitro activity of TIF-3C5 - inhibits IFNalpha -induced (IFNalpha-A, -1, -4, -5, -11 and 13) Stat1 phosphorylation in vitro. [TIF-1D6 does not block in vitro functional activity]. - blocks IFNA (IFNA-A, -1, -4, -5, -11 and 13) induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines in a dose dependent manner. - neutralizes IFNA-induced antiviral activity in vitro following infection of L929 cells with VSV. Functional activity of both recombinant (IFNalpha-A, -1, -4, -5, -11 and 13) and natural IFNalpha can be blocked with no neutralization of IFNgor IFNb. In vivo activity of TIF-3C5 - TIF-3C5 circulates with a half-life of 4 days - blockade of IFNa by TIF-3C5 increases the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of Irf7-/- mice.

Other Notes

Small volumes of anti-IFNalpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IFNalpha. It may not necessarily be applicable to this product.

NCBI GI #
117168293
NCBI GeneID
15962
NCBI Accession #
NP_034632.2
UniProt Primary Accession #
P01572
UniProt Secondary Accession #
Q7M0A3; Q810G7
UniProt Related Accession #
P01572
GenBank Nucleotide #
NM_010502.2
NCBI Official Full Name
interferon alpha-1
NCBI Official Synonym Full Names
interferon alpha 1
NCBI Official Symbol
Ifna1
NCBI Official Synonym Symbols
Ifa1
UniProt Protein Name
Interferon alpha-1
Protein Family
Interferon
UniProt Gene Name
Ifna1
UniProt Synonym Gene Names
IFN-alpha-1
UniProt Entry Name
IFNA1_MOUSE
NCBI Protein Information
interferon alpha-1; IFN-alpha-1; interferon alpha family gene 1

UniProt Comments for IFNalpha

IFNA1/13: a cytokine that belongs to the alpha/beta interferon family.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity

Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; B cell differentiation; cytokine and chemokine mediated signaling pathway; defense response to bacterium; natural killer cell activation during immune response; regulation of MHC class I biosynthetic process; innate immune response; defense response; defense response to virus; humoral immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; T cell activation during immune response
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Research Articles on IFNalpha

1. IL-12 and IFN-alpha differentially program CD8 T cells to re-express distinct levels of PD-1 upon re-encountering Ag, resulting in IL-12-stimulated cells being less susceptible to exhaustion in the face of sustained tumor Ag.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IFNalpha antibody

 Reference Product  PubMed Publications
 Ifnb1 antibody  >7 publications with IFNalpha and Ifnb1
 Irf7 antibody  >6 publications with IFNalpha and Irf7
 Ifnar1 antibody  >2 publications with IFNalpha and Ifnar1
 Irf5 antibody  >1 publications with IFNalpha and Irf5

Pathways associated with anti-IFNalpha antibody

 Products by Pathway  Pathway Diagram
 Autoimmune Thyroid Disease Pathway antibodies  Autoimmune Thyroid Disease Pathway Diagram
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 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response (BioCarta) Pathway antibodies  Cytokines And Inflammatory Response (BioCarta) Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
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 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram

Organs/Tissues associated with anti-IFNalpha antibody

 Organ/Tissue Name  Pubmed Publications
 Spleen Antibodies  >1 publications with IFNalpha and Spleen
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