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Alpha Fetoprotein (AFP) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS310966

MBS310966 is an antibody targeting AFP. Also known as MAb to Human Alpha Fetoprotein. Host: Mouse. Hybridization of P3X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice.Source: Ascites. More details

$910 / 1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Alpha Fetoprotein (AFP), Monoclonal Antibody
Full Product Name
MAb to Human Alpha Fetoprotein
Product Gene Name
anti-AFP antibody
Product Synonym Names
Monoclonal Antibody to Human Alpha Fetoprotein (AFP)
Clonality
Monoclonal
Isotype
IgG1
Host
Host: Mouse. Hybridization of P3X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice.
Source: Ascites
Form/Format
Purified, Liquid
Purity/Purification
Prosep A purified
Concentration
3.02 mg/ml (OD280nm, E^1% = 14) (lot specific)
Specificity
Alpha Fetoprotein (AFP)
No cross-reactivity with Human Albumin, CEA, Ferritin, hCG, PSA.
Preparation and Storage
Short-term (up to 6 months) store at 2 to 8 degree C. Long term, aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles.
Molecular Weight
68,678 Da
Chromosome Location
chromosome: 4; Location: 4q11-q13
OMIM
104150
3D Structure
ModBase 3D Structure for P02771
Immunogen
Purified AFP from Cord Blood
Affinity Constant
5 x 10^9
Buffer
PBS, pH 7.4 +/- 0.1
Preservative
0.1% Sodium azide
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

Product Categories/Family for anti-AFP antibody

Monoclonal Antibodies for Cancer Research
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-AFP antibody
Immunoassay Antibody Pairs
Application Notes for anti-AFP antibody
For enzyme conjugation, radio labeling or solid phase immobilization. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Capture: MBS310966; Detection: MBS310949

Other Notes

Small volumes of anti-AFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AFP. It may not necessarily be applicable to this product.

NCBI GI #
178236
NCBI GeneID
174
NCBI Accession #
AAB58754.1
UniProt Primary Accession #
P02771
UniProt Secondary Accession #
B2RBU3
UniProt Related Accession #
P02771
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Synonym Full Names
alpha-fetoprotein
NCBI Official Symbol
AFP
NCBI Official Synonym Symbols
FETA; HPAFP; AFP
UniProt Protein Name
Alpha-fetoprotein
UniProt Synonym Protein Names
Alpha-1-fetoprotein; Alpha-fetoglobulin
Protein Family
Alpha-fetoprotein
UniProt Gene Name
AFP
UniProt Synonym Gene Names
HPAFP
UniProt Entry Name
FETA_HUMAN
NCBI Protein Information
alpha-fetoprotein

NCBI Summary for AFP

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq]
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UniProt Comments for AFP

AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cytoplasm

Molecular Function: metal ion binding

Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle

Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of
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Research Articles on AFP

1. Assessment of Dkk3 and AFP may be useful in the diagnosis of hepatic tumors.
2. AFP promoter enhancer increased specific expression of the human sodium iodide symporter (hNIS) for targeted radioiodine therapy of hepatocellular carcinoma.
3. Alpha-fetoprotein was more sensitive than des-gamma carboxyprothrombin and lectin-bound alpha-fetoprotein in diagnosing early hepatocellular carcinoma.
4. Case Report: Alpha-fetoprotein (AFP)-producing ovarian tumor in an elderly woman.
5. The utility of the tumour markers carcinoembryonic antigen, cancer antigen 125, carbohydrate antigen 19-9, carbohydrate antigen 15-3, alpha-fetoprotein and human chorionic gonadotropin for the diagnosis solitary pulmonary nodules, was investigated.
6. Case Report: Alpha-fetoprotein expressing metastastic adenocarcinoma of the esophago-gastric junction responding favorably to capecitabine and oxaliplatin.
7. small amplitude undulations in AFP are associated with recurrence of germ cell neoplasm
8. Low Alphafetoprotein levels and increased CA 19-9 levels can be used as tumor markers in diagnosis of molar pregnancies in conjunction with clinical and sonographic findings and serum levels of human chorionic gonadotropin
9. Report des-gamma-carboxyprothrombin, alpha-fetoprotein and AFP-L3 in patients with chronic hepatitis, cirrhosis and hepatocellular carcinoma.
10. These data support the idea that ZHX2 contributes to AFP repression in the liver after birth and may also be involved in AFP reactivation in liver cancer.
11. In hepatocellular carcinoma, pre-transplant AFP levels >400 ng/ml were associated with higher tumor recurrence.
12. Serum samples were investigated for carcinoembryonic antigen (CEA), CA125 and MUC1, alpha-foetoprotein, neuron-specific enolase and CA19.9.
13. In patients with hepatocellular carcinoma, a high frequency of AFP-specific CD8(+) T cells directed against different epitopes suggest that AFP has a strong and broad immunogenicity.
14. Molecular background of AFP in liver cancer cells as revealed by global RNA expression analysis is reported.
15. results suggest that AFP may positively regulate cell proliferation by enhancing the apoptosis resistance via dysfunction of the p53/Bax/cytochrome c/caspase-3 signaling pathway in AFP
16. Elevated alpha-fetoprotein is associated with primary central nervous system germ cell tumors.
17. Results indicate that the poor prognosis associated with high alpha-fetoprotein in hepatocellular carcinoma is due to high cell proliferation, high angiogenesis, and low apoptosis.
18. targeting of AFP with siRNA could be a potential therapeutic approach for hepatoma
19. substitution of aa 70 of HCV-1b core region is an important predictor of elevated AFP in non-HCC (hepatocellular carcinoma) patients.
20. The N-glycan structures of the Lens culinaris agglutinin (LCA)-reactive fraction of alpha-fetoprotein (AFP-L3), a tumor marker of hepatocellular carcinomas (HCC), were analyzed.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AFP antibody

 Reference Product  PubMed Publications
 CCR5 antibody  >2 publications with AFP and CCR5

Pathways associated with anti-AFP antibody

 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram

Diseases associated with anti-AFP antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >4487 publications with AFP and Neoplasms
 Carcinoma Antibodies  >3321 publications with AFP and Carcinoma
 Liver Diseases Antibodies  >3093 publications with AFP and Liver Diseases
 Liver Neoplasms Antibodies  >3033 publications with AFP and Liver Neoplasms
 Adenocarcinoma Antibodies  >2675 publications with AFP and Adenocarcinoma
 Carcinoma, Hepatocellular Antibodies  >2652 publications with AFP and Carcinoma, Hepatocellular
 Hepatitis Antibodies  >872 publications with AFP and Hepatitis
 Liver Cirrhosis Antibodies  >837 publications with AFP and Liver Cirrhosis
 Congenital Abnormalities Antibodies  >730 publications with AFP and Congenital Abnormalities
 Male Urogenital Diseases Antibodies  >638 publications with AFP and Male Urogenital Diseases

Organs/Tissues associated with anti-AFP antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >3740 publications with AFP and Liver
 Blood Antibodies  >3032 publications with AFP and Blood
 Vascular Antibodies  >395 publications with AFP and Vascular
 Lung Antibodies  >334 publications with AFP and Lung
 Brain Antibodies  >294 publications with AFP and Brain
 Embryonic Tissue Antibodies  >260 publications with AFP and Embryonic Tissue
 Muscle Antibodies  >163 publications with AFP and Muscle
 Kidney Antibodies  >161 publications with AFP and Kidney
 Placenta Antibodies  >153 publications with AFP and Placenta
 Heart Antibodies  >92 publications with AFP and Heart
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