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RUNX3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9211869

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can… More details

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Product Overview

Online Datasheet in PDF
Product Name
RUNX3, Polyclonal Antibody
Full Product Name
RUNX3 Antibody (Center)
Product Gene Name
anti-RUNX3 antibody
Product Synonym Names
Runt-related transcription factor 3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEBP2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; RUNX3; AML2; CBFA3; PEBP2A3
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Specificity
This RUNX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human RUNX3.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
RUNX3 peptide (MBS9220995) is used for blocking the activity of RUNX3 antibody (MBS9211869)
Immunogen Sequence Positions
168-197
OMIM
600210
3D Structure
ModBase 3D Structure for Q13761
Function
Forms the heterodimeric complexcore-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the coreconsensus binding sequence 5'- TGTGGT-3',or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain,while CBFB is a non-DNA-binding regulatorysubunit that allosterically enhances thesequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters,including murine leukemia virus,polyomavirus enhancer, T-cell receptorenhancers, LCK, IL3 and GM-CSF promoters(By similarity). May be involved in the controlof cellular proliferation and/or differentiation. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-betastimulation (PubMed: 20599712). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).
Cellular Location
Nucleus{ECO:0000255|PROSITE-ProRule: PRU00399,ECO:0000269|PubMed:20100835,ECO:0000269|PubMed:20599712}.Cytoplasm. Note=The tyrosine phosphorylated form localizes to the cytoplasm. Translocates from the cytoplasm to the nucleus following TGF-beta stimulation
Tissue Location
Expressed in gastric cancer tissues (at protein level).

Product Categories/Family for anti-RUNX3 antibody

Crown Antibodies
Applications Tested/Suitable for anti-RUNX3 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-RUNX3 antibody
IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50

Western Blot (WB) of anti-RUNX3 antibody

Western blot analysis of lysates from HL-60, Jurkat cell line (from left to right), using RUNX3 Antibody (Center). MBS9211869 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Rabbit Anti-RUNX3 Polyclonal Antibody – Cat# MBS9211869

Immunohistochemistry (IHC) of anti-RUNX3 antibody

RUNX3 Antibody (Center) (MBS9211869)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-RUNX3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9211869

Immunofluorescence (IF) of anti-RUNX3 antibody

Fluorescent confocal image of Hela cell stained with RUNX3 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RUNX3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RUNX3 immunoreactivity is localized to Nucleus and Cytoplasm significantly.
Rabbit Anti-RUNX3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9211869

Product References and Citations for anti-RUNX3 antibody

Tsang, Y.H., et al. Oncogene 29(41):5643-5650(2010)
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Iwatani, K., et al. Biochem. Biophys. Res. Commun. 400(3):426-431(2010)
Kodach, L.L., et al. Carcinogenesis 31(9):1567-1575(2010)
Nishio, M., et al. Anticancer Res. 30(7):2673-2682(2010)

Other Notes

Small volumes of anti-RUNX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RUNX3 purchase

MBS9211869 is an antibody targeting RUNX3. Also known as RUNX3 Antibody (Center). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RUNX3. It may not necessarily be applicable to this product.

NCBI GI #
72534652
NCBI GeneID
864
NCBI Accession #
NP_001026850.1
NCBI Related Accession #
NP_004341.1
UniProt Primary Accession #
Q13761
UniProt Secondary Accession #
Q12969; Q13760; B1AJV5
UniProt Related Accession #
Q13761
GenBank Nucleotide #
NM_001031680.2
NCBI Official Full Name
runt-related transcription factor 3 isoform 1
NCBI Official Synonym Full Names
runt-related transcription factor 3
NCBI Official Symbol
RUNX3
NCBI Official Synonym Symbols
AML2; CBFA3; PEBP2aC
UniProt Protein Name
Runt-related transcription factor 3
UniProt Synonym Protein Names
Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEBP2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit
Protein Family
Runt-related transcription factor
UniProt Gene Name
RUNX3
UniProt Synonym Gene Names
AML2; CBFA3; PEBP2A3; CBF-alpha-3; PEA2-alpha C; PEBP2-alpha C
UniProt Entry Name
RUNX3_HUMAN
NCBI Protein Information
runt-related transcription factor 3

UniProt Comments for RUNX3

AML2: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA- binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; nuclear chromatin; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; transcription factor activity; ATP binding

Biological Process: transcription from RNA polymerase II promoter; peripheral nervous system neuron development; axon guidance; hair follicle morphogenesis; regulation of transcription, DNA-dependent; cell maturation; interferon-gamma production; chondrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; negative regulation of cell cycle; negative regulation of epithelial cell proliferation
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Research Articles on RUNX3

1. RUNX3 inactivation by promoter hypermethylation plays a role in the progression of malignant transformation of ovarian endometriosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RUNX3 antibody

 Reference Product  PubMed Publications
 CCND1 antibody  >16 publications with RUNX3 and CCND1
 SMAD4 antibody  >16 publications with RUNX3 and SMAD4
 ZBTB7B antibody  >12 publications with RUNX3 and ZBTB7B
 EZH2 antibody  >12 publications with RUNX3 and EZH2
 CDKN1A antibody  >11 publications with RUNX3 and CDKN1A
 CBFB antibody  >9 publications with RUNX3 and CBFB
 SMAD3 antibody  >8 publications with RUNX3 and SMAD3
 SP7 antibody  >2 publications with RUNX3 and SP7
 NTRK3 antibody  >1 publications with RUNX3 and NTRK3

Pathways associated with anti-RUNX3 antibody

 Products by Pathway  Pathway Diagram
 Synthesis And Degradation Of Ketone Bodies Pathway antibodies  Synthesis And Degradation Of Ketone Bodies Pathway Diagram
 Synthesis And Degradation Of Ketone Bodies Pathway antibodies  Synthesis And Degradation Of Ketone Bodies Pathway Diagram
 Synthesis And Degradation Of Ketone Bodies Pathway antibodies  Synthesis And Degradation Of Ketone Bodies Pathway Diagram

Diseases associated with anti-RUNX3 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >217 publications with RUNX3 and Carcinoma
 Stomach Neoplasms Antibodies  >128 publications with RUNX3 and Stomach Neoplasms
 Adenocarcinoma Antibodies  >117 publications with RUNX3 and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >41 publications with RUNX3 and Carcinoma, Squamous Cell
 Neoplasm Metastasis Antibodies  >41 publications with RUNX3 and Neoplasm Metastasis
 Lung Diseases Antibodies  >40 publications with RUNX3 and Lung Diseases
 Liver Neoplasms Antibodies  >38 publications with RUNX3 and Liver Neoplasms
 Lung Neoplasms Antibodies  >35 publications with RUNX3 and Lung Neoplasms
 Liver Diseases Antibodies  >34 publications with RUNX3 and Liver Diseases
 Inflammation Antibodies  >34 publications with RUNX3 and Inflammation

Organs/Tissues associated with anti-RUNX3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >190 publications with RUNX3 and Blood
 Stomach Antibodies  >142 publications with RUNX3 and Stomach
 Bone Antibodies  >78 publications with RUNX3 and Bone
 Lung Antibodies  >60 publications with RUNX3 and Lung
 Lymph Antibodies  >39 publications with RUNX3 and Lymph
 Lymph Node Antibodies  >38 publications with RUNX3 and Lymph Node
 Thymus Antibodies  >33 publications with RUNX3 and Thymus
 Intestine Antibodies  >32 publications with RUNX3 and Intestine
 Ganglia Antibodies  >28 publications with RUNX3 and Ganglia
 Brain Antibodies  >23 publications with RUNX3 and Brain
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