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POLE4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3204597

This is a rabbit polyclonal antibody against POLE4. It was validated on Western Blot using a cell lysate as a positive control.Target Description: POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a… More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLE4, Polyclonal Antibody
Full Product Name
POLE4 antibody - N-terminal region
Product Gene Name
Product Synonym Gene Name
p12; YHHQ1
Immunogen Sequence
Synthetic peptide located within the following region: MAAAAAAGSG TPREEEGPAG EAAASQPQAP TSVPGARLSR LPLARVKALV
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
POLE4 peptide (MBS3229564) is used for blocking the activity of POLE4 antibody (MBS3204597)
Molecular Weight
12kDa
OMIM
607269
3D Structure
ModBase 3D Structure for Q9NR33
Homology
Cow: 86%; Dog: 93%; Guinea Pig: 93%; Horse: 86%; Human: 100%; Rat: 79%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human POLE4
Applications Tested/Suitable for anti-POLE4 antibody
Western Blot (WB)

Western Blot (WB) of anti-POLE4 antibody

WB Suggested Anti-POLE4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: COLO205 cell lysate
Rabbit Anti-POLE4 Polyclonal Antibody – Cat# MBS3204597

Other Notes

Small volumes of anti-POLE4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLE4 purchase

MBS3204597 is an antibody targeting POLE4. Also known as POLE4 antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POLE4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA polymerase epsilon subunit 4
NCBI Official Synonym Full Names
DNA polymerase epsilon 4, accessory subunit
NCBI Official Symbol
POLE4
NCBI Official Synonym Symbols
p12; YHHQ1
UniProt Protein Name
DNA polymerase epsilon subunit 4
UniProt Synonym Protein Names
DNA polymerase II subunit 4; DNA polymerase epsilon subunit p12
Protein Family
UniProt Gene Name
POLE4
UniProt Entry Name
DPOE4_HUMAN
NCBI Protein Information
DNA polymerase epsilon subunit 4

NCBI Summary for POLE4

POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]

UniProt Comments for POLE4

POLE4: May play a role in allowing polymerase epsilon to carry out its replication and/or repair function.

Protein type: Nucleotide Metabolism - pyrimidine; EC 2.7.7.7; Transferase; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: epsilon DNA polymerase complex; nucleus

Molecular Function: protein binding; protein heterodimerization activity; sequence-specific DNA binding; DNA-directed DNA polymerase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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