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SMYD3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3210038

This is a rabbit polyclonal antibody against SMYD3. It was validated on Western Blot using a cell lysate as a positive control.Target Description: SMYD3 is a member of an RNA polymerase complex. Within the RNA polymerase complex, it acts as a histone… More details

$490 / 0.1 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
SMYD3, Polyclonal Antibody
Full Product Name
SMYD3 antibody - N-terminal region
Product Gene Name
anti-SMYD3 antibody
Product Synonym Gene Name
FLJ21080; MGC104324; ZMYND1; ZNFN3A1; bA74P14.1; KMT3E
Immunogen Sequence
Synthetic peptide located within the following region: PRYPPDSVRL LGRVVFKLMD GAPSESEKLY SFYDLESNIN KLTEDKKEGL
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
SMYD3 peptide (MBS3234993) is used for blocking the activity of SMYD3 antibody (MBS3210038)
Molecular Weight
42kDa
OMIM
608783
3D Structure
ModBase 3D Structure for Q9H7B4
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human SMYD3

Product Categories/Family for anti-SMYD3 antibody

Polyclonal; Chromatin & Nuclear Signaling
Applications Tested/Suitable for anti-SMYD3 antibody
Chromatin IP (ChIP), Western Blot (WB)

Chromatin Immunoprecipitation (ChIP) of anti-SMYD3 antibody

Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Rabbit Anti-SMYD3 Polyclonal Antibody – Cat# MBS3210038

Western Blot (WB) of anti-SMYD3 antibody

Host: Rabbit
Target Name: SMYD3
Sample Tissue: Human Ovary Tumor
Antibody Dilution: 1ug/ml
Rabbit Anti-SMYD3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3210038

Western Blot (WB) of anti-SMYD3 antibody

WB Suggested Anti-SMYD3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Stomach
Rabbit Anti-SMYD3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3210038

Other Notes

Small volumes of anti-SMYD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMYD3 purchase

MBS3210038 is an antibody targeting SMYD3. Also known as SMYD3 antibody - N-terminal region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SMYD3. It may not necessarily be applicable to this product.

NCBI GI #
12232401
NCBI GeneID
64754
NCBI Accession #
NP_073580
UniProt Primary Accession #
Q9H7B4
UniProt Related Accession #
Q9H7B4
GenBank Nucleotide #
NM_022743
NCBI Official Full Name
histone-lysine N-methyltransferase SMYD3 isoform 2
NCBI Official Synonym Full Names
SET and MYND domain containing 3
NCBI Official Symbol
SMYD3
NCBI Official Synonym Symbols
KMT3E; ZMYND1; ZNFN3A1; bA74P14.1
UniProt Protein Name
Histone-lysine N-methyltransferase SMYD3
UniProt Synonym Protein Names
SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1
Protein Family
Histone-lysine N-methyltransferase
UniProt Gene Name
SMYD3
UniProt Synonym Gene Names
ZMYND1; ZNFN3A1
UniProt Entry Name
SMYD3_HUMAN
NCBI Protein Information
histone-lysine N-methyltransferase SMYD3

NCBI Summary for SMYD3

This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

UniProt Comments for SMYD3

SMYD3: Histone methyltransferase. Specifically methylates 'Lys- 4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. Belongs to the histone-lysine methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: metal ion binding; histone-lysine N-methyltransferase activity

Biological Process: nucleosome assembly; establishment and/or maintenance of chromatin architecture; establishment of protein localization; myotube cell development; negative regulation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation
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Research Articles on SMYD3

1. Study reported for the first time that SMYD3 promoter hypomethylation was associated with colorectal cancer in a Chinese cohort.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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