• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

KMT5B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3210270

This is a rabbit polyclonal antibody against Suv420h1. It was validated on Western Blot More details

$490 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KMT5B, Polyclonal Antibody
Full Product Name
KMT5B Antibody - C-terminal region
Product Gene Name
anti-KMT5B antibody
Product Synonym Gene Name
AA117471; Suv420h1; Suv4-20h1; C630029K18Rik
Immunogen Sequence
Synthetic peptide located within the following region: FINHDCRPNC KFVSTGRDTA CVKALRDIEP GEEISCYYGD GFFGENNEFC
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Tested: Mouse
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Concentration
Batch dependent within range: 0.5-1 mg/ml (lot specific)
Preparation and Storage
For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
KMT5B peptide (MBS3235226) is used for blocking the activity of KMT5B antibody (MBS3210270)
Molecular Weight
35kDa
3D Structure
ModBase 3D Structure for Q3U8K7
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 77%; Rat: 100%; Zebrafish: 77%
Protein Size
327 amino acids

Product Categories/Family for anti-KMT5B antibody

Polyclonal; Transcription Regulation; Epigenetics; Chromatin Immunoprecipitation; Methylation; Transcription Factors
Applications Tested/Suitable for anti-KMT5B antibody
Chromatin IP (ChIP), Western Blot (WB)

Chromatin Immunoprecipitation (ChIP) of anti-KMT5B antibody

Chromatin Immunoprecipitation (ChIP) Using Suv420h1 antibody - C-terminal region and HCT116 Cells
Rabbit Anti-KMT5B Polyclonal Antibody – Cat# MBS3210270

Chromatin Immunoprecipitation (ChIP) of anti-KMT5B antibody

Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
Rabbit Anti-KMT5B Polyclonal Antibody, Chromatin Immunoprecipitation (ChIP) – Cat# MBS3210270

Western Blot (WB) of anti-KMT5B antibody

WB Suggested Anti-Suv420h1 Antibody
Titration: 1.0 ug/ml
Positive Control: Mouse Thymus
Rabbit Anti-KMT5B Polyclonal Antibody, Western Blot (WB) – Cat# MBS3210270

Other Notes

Small volumes of anti-KMT5B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KMT5B purchase

MBS3210270 is an antibody targeting KMT5B. Also known as KMT5B Antibody - C-terminal region. Reactive in Tested: Mouse
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KMT5B. It may not necessarily be applicable to this product.

NCBI GI #
268607617
NCBI GeneID
225888
NCBI Accession #
NP_001161356
UniProt Primary Accession #
Q3U8K7
UniProt Related Accession #
Q3U8K7
GenBank Nucleotide #
NM_001167884
NCBI Official Full Name
histone-lysine N-methyltransferase KMT5B isoform d
NCBI Official Synonym Full Names
lysine methyltransferase 5B
NCBI Official Symbol
Kmt5b
NCBI Official Synonym Symbols
AA117471; Suv420h1; Suv4-20h1; C630029K18Rik
UniProt Protein Name
Histone-lysine N-methyltransferase SUV420H1
UniProt Synonym Protein Names
Suppressor of variegation 4-20 homolog 1
Protein Family
Histone-lysine N-methyltransferase
UniProt Gene Name
Suv420h1
UniProt Synonym Gene Names
Su(var)4-20 homolog 1; Suv4-20h1
UniProt Entry Name
SV421_MOUSE
NCBI Protein Information
histone-lysine N-methyltransferase KMT5B

UniProt Comments for KMT5B

SUV420H1: a protein methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Strongly down-regulated in breast cancer cells. Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. Three alternatively-spliced isoforms have been described.

Protein type: EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; Methyltransferase

Cellular Component: chromosome; nucleus; condensed nuclear chromosome, pericentric region

Molecular Function: transferase activity; methyltransferase activity; histone lysine N-methyltransferase activity (H4-K20 specific); histone-lysine N-methyltransferase activity

Biological Process: histone methylation; methylation; muscle development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin modification; peptidyl-lysine methylation
Show more

Research Articles on KMT5B

1. Suv4-20h1/h2 are mostly absent in mouse embryos before implantation, underscoring a rapid decrease of H4K20me3 from the two-cell stage onward

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-KMT5B antibody

 Products by Pathway  Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram

Diseases associated with anti-KMT5B antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with KMT5B and Nervous System Diseases

Organs/Tissues associated with anti-KMT5B antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with KMT5B and Muscle
 Brain Antibodies  >1 publications with KMT5B and Brain
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000