Rabbit Anti-GTF2A1 Polyclonal Antibody – Cat# MBS3213767

WB Suggested Anti-GTF2A1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: HepG2 cell lysate

GTF2A1 Antibody – Rabbit Polyclonal

Name: GTF2A1 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS3213767
Price: 490 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3213767

This is a rabbit polyclonal antibody against GTF2A1. It was validated on Western BlotTarget Description: Transcription initiation factor IIA . and . chains (GTF2A1, TFIIA p35 and p19 subunits, TFIIAL) binding ability of TLP is required for characteristic… More details

$490 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GTF2A1, Polyclonal Antibody

Full Product Name

GTF2A1 antibody - middle region

Product Gene Name

anti-GTF2A1 antibody

Product Synonym Gene Name

MGC129969; MGC129970; TF2A1; TFIIA

Immunogen Sequence

Synthetic peptide located within the following region: GQQQPQAQPA QTQAPLVLQV DGTGDTSSEE DEDEEEDYDD DEEEDKEKDG

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Yeast

Form/Format

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Purity/Purification

Affinity Purified

Preparation and Storage

For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Antibody/Peptide Pairs

GTF2A1 peptide (MBS3238706) is used for blocking the activity of GTF2A1 antibody (MBS3213767)

Molecular Weight

37kDa

OMIM

600520

3D Structure

ModBase 3D Structure for P52655

Homology

Cow: 100%; Dog: 93%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Yeast: 93%

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human GTF2A1

Product Categories/Family for anti-GTF2A1 antibody

Polyclonal; Transcription Factor; Transcription Regulation; Cell Biology; Various; Transcription Factors

Applications Tested/Suitable for anti-GTF2A1 antibody

Western Blot (WB)

Other Notes

Small volumes of anti-GTF2A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GTF2A1 purchase

MBS3213767 is an antibody targeting GTF2A1. Also known as GTF2A1 antibody - middle region. Reactive in Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Yeast. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GTF2A1. It may not necessarily be applicable to this product.

NCBI GI #

42476101

NCBI GeneID

2957

NCBI Accession #

NP_963889

UniProt Primary Accession #

P52655

UniProt Related Accession #

P52655

GenBank Nucleotide #

NM_201595

NCBI Official Full Name

transcription initiation factor IIA subunit 1 isoform 2

NCBI Official Synonym Full Names

general transcription factor IIA subunit 1

NCBI Official Symbol

GTF2A1

NCBI Official Synonym Symbols

TF2A1; TFIIA; TFIIAL; TFIIA-42

UniProt Protein Name

Transcription initiation factor IIA subunit 1

UniProt Synonym Protein Names

General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42Cleaved into the following 2 chains:Transcription initiation factor IIA alpha chain; Alternative name(s):; TFIIA p35 subunitTranscription initiation factor IIA beta chain; Alternative name(s):; TFIIA p19 subunit

Protein Family

Transcription initiation factor

UniProt Gene Name

GTF2A1

UniProt Synonym Gene Names

TF2A1; TFIIA-42

UniProt Entry Name

TF2AA_HUMAN

NCBI Protein Information

transcription initiation factor IIA subunit 1

NCBI Summary for GTF2A1

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]

UniProt Comments for GTF2A1

GTF2A1: a general transcription factor that plays an important role in transcriptional activation. A component of the transcription machinery of RNA polymerase II. Phosphorylated by Taf(II) 250 on serines that regulate TBP binding. Interacts with TBP (the TATA-binding protein). Two isoforms, 42 kDa and 37 kDa, are produced by alternative initiation.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 14q31.1

Cellular Component: nucleoplasm; cytoplasm; transcription factor TFIIA complex

Molecular Function: protein binding; DNA binding; TATA-binding protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; viral reproduction; RNA elongation from RNA polymerase II promoter; gene expression

Research Articles on GTF2A1

1. In a cohort of youth at risk for bipolar disorder, pathway analysis showed an enrichment of the glucocorticoid receptor (GR) pathway with the genes MED1, HSPA1L, GTF2A1 and TAF15, which might underlie the previously reported role of stress response in the risk for bipolar disorder in vulnerable populations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.