DOC2B Antibody – Rabbit Polyclonal

Function: Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes

By similarity. Ref.2

Subunit structure: Interacts with the SNARE (soluble N-ethylmaleimide-sensitive factor attached protein receptor) complex composed of SNAP25, STX1A and VAMP2; the interaction is calcium-dependent and competitive with SYT1. Interacts with STX4; the interaction is calcium-dependent, increased by insulin and glucose, and mediates vesicle fusion with plasma membrane in pancreatic cells and adipocytes. Interacts with STXBP3; the interaction is direct, occurs at the cell membrane and regulates glucose-stimulated insulin secretion. May interact with UNC13A; the interaction mediates targeting to the plasma membrane

By similarity. Interacts with cytoplasmic dynein light chain DYNLT1. Ref.2

Subcellular location: Cytoplasm. Cytoplasmic granule

By similarity. Cell membrane; Peripheral membrane protein

By similarity. Note: Translocates to the plasma membrane in a calcium-dependent manner

By similarity.

Tissue specificity: Widely expressed with highest levels in brain and kidney. Expressed in pancreatic islet cells (at protein level). Ref.1 Ref.3

Domain: C2 domain 1 is involved in binding calcium and phospholipids. C2 domain 2 may also play a role in the calcium-dependent targeting to membranes

By similarity.

Sequence similarities: Contains 2 C2 domains.