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Matrix Metalloproteinase 8 (MMP-8) ELISA Kit for Horse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS032337

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Matrix Metalloproteinase 8 (MMP-8), ELISA Kit
Full Product Name
Horse Matrix Metalloproteinase 8 ELISA Kit
Product Gene Name
Species Reactivity
Horse
Assay Type
Sandwich
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Detection Range
3.12 ng/ml - 100 ng/ml.
Sensitivity
1.0 ng/ml.
Specificity
No significant cross-reactivity or interference between Horse MMP-8 and analogues was observed.
Preparation and Storage
Store all reagents at 2-8 degree C
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Molecular Weight
53,412 Da
Chromosome Location
Chromosome: 11; NC_000011.9 (102582526..102595685, complement). Location: 11q22.3
OMIM
120355
3D Structure
ModBase 3D Structure for P22894
Intended Uses
ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate MMP-8 concentrations in Horse serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic applications

Typical Testing Data/Standard Curve (for reference only) of MMP-8 elisa kit

Horse MMP-8 ELISA Kit – Cat# MBS032337

Other Notes

Small volumes of MMP-8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP-8 purchase

MBS032337 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Matrix Metalloproteinase 8 (MMP-8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MMP-8. The ELISA analytical biochemical technique of the MBS032337 kit is based on MMP-8 antibody-MMP-8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MMP-8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MMP-8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MMP-8. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
matrix metalloproteinase 8 (neutrophil collagenase)
NCBI Official Synonym Full Names
matrix metallopeptidase 8 (neutrophil collagenase)
NCBI Official Symbol
MMP8
NCBI Official Synonym Symbols
HNC; CLG1; MMP-8; PMNL-CL
UniProt Protein Name
Neutrophil collagenase
UniProt Synonym Protein Names
Matrix metalloproteinase-8; MMP-8; PMNL collagenase
UniProt Gene Name
MMP8
UniProt Synonym Gene Names
CLG1; MMP-8; PMNL-CL
UniProt Entry Name
MMP8_HUMAN
NCBI Protein Information
neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase)

NCBI Summary for MMP-8

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
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UniProt Comments for MMP-8

MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.

Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.24.34

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity; calcium ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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