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Musculin (MSC) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9600277

Description: MSC Transcription repressor capable of inhibiting the transactivation capability of TCF3/E47. May play a role in regulating antigen-dependent B-cell differentiation. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Musculin (MSC), Polyclonal Antibody
Full Product Name
Musculin Antibody
Product Gene Name
Product Synonym Names
ABF-1; ABF1; Activated B cell factor 1; Activated B-cell factor 1; bHLHa22; Class A basic helix-loop-helix protein 22; Msc; MUSC_HUMAN; Musculin; MYOR
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
Musculin antibody detects endogenous levels of total Musculin
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
Molecular Weight
Observed: 22 kDa
Predicted: 23 kDa
3D Structure
ModBase 3D Structure for O60682
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Musculin
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in lymphoid tissues, B-cell lines and activated B-cells.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-MSC antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-MSC antibody
WB: 1:500-1:3000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-MSC antibody

Western blot analysis on rat brain lysate using Musculin Antibody
Rabbit Anti-MSC Polyclonal Antibody – Cat# MBS9600277

Immunohistochemistry (IHC) of anti-MSC antibody

MBS9600277 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-MSC Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9600277

Immunofluorescence (IF) of anti-MSC antibody

MBS9600277 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-MSC Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9600277

Western Blot (WB) of anti-MSC antibody

Western blot analysis on Jurkat cell lysate using Musculin Antibody, The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-MSC Polyclonal Antibody, Western Blot (WB) – Cat# MBS9600277

Other Notes

Small volumes of anti-MSC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MSC purchase

MBS9600277 is an antibody targeting MSC. Also known as Musculin Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MSC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
musculin
NCBI Official Synonym Full Names
musculin
NCBI Official Symbol
MSC
NCBI Official Synonym Symbols
ABF1; MYOR; ABF-1; bHLHa22
UniProt Protein Name
Musculin
UniProt Synonym Protein Names
Activated B-cell factor 1; ABF-1; Class A basic helix-loop-helix protein 22; bHLHa22
Protein Family
UniProt Gene Name
MSC
UniProt Synonym Gene Names
ABF1; BHLHA22; ABF-1; bHLHa22
NCBI Protein Information
musculin

NCBI Summary for MSC

The protein encoded by this gene is a transcriptional repressor capable of binding an E-box element either as a homodimer or as a heterodimer with E2A in vitro. The encoded protein also forms heterodimers with E2A proteins in vivo. This protein is capable of inhibiting the transactivation capability of E47, an E2A protein, in mammalian cells. This gene is a downstream target of the B-cell receptor signal transduction pathway. [provided by RefSeq, Jul 2008]
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UniProt Comments for MSC

Transcription repressor capable of inhibiting the transactivation capability of TCF3/E47. May play a role in regulating antigen-dependent B-cell differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MSC antibody

Pathways associated with anti-MSC antibody

 Products by Pathway  Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
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