Mouse Anti-KRT18 Monoclonal Antibody – Cat# MBS346010

IHC (paraffin) of human colon using Cytokeratin 18 Antibody

Cytokeratin 18 (KRT18) Antibody – Mouse Monoclonal

Name: KRT18 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS346010
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS346010

CK18 (Cytokeratin 18) is part of the type I keratin family proteins, and usually forms multimers with its partner cytokeratin 8. They can be found in simple, glandular and transitional epithelial cells, but are absent in stratified epithelial cells.… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Cytokeratin 18 (KRT18), Monoclonal Antibody

Full Product Name

Cytokeratin 18 Antibody

Product Gene Name

anti-KRT18 antibody

Clonality

Monoclonal

Isotype

IgG

Clone Number

IHC018

Host

Mouse

Species Reactivity

Human

Form/Format

Liquid

Preparation and Storage

Store at 2-8 degree C. Do not freeze.

Molecular Weight

48,058 Da

3D Structure

ModBase 3D Structure for P05783

Buffer

Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3

IHC Positive Control

Breast, Breast Carcinoma

IHC Suggested Protocol

1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block – Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)

Applications Tested/Suitable for anti-KRT18 antibody

Immunohistochemistry (IHC) Paraffin

Application Notes for anti-KRT18 antibody

Dilution Range: 1:100 - 1:200

Other Notes

Small volumes of anti-KRT18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT18 purchase

MBS346010 is an antibody targeting KRT18. Also known as Cytokeratin 18 Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT18. It may not necessarily be applicable to this product.

NCBI GI #

34037

NCBI GeneID

3875

NCBI Accession #

CAA31375.1

UniProt Primary Accession #

P05783

UniProt Secondary Accession #

Q53G38; Q5U0N8; Q9BW26

UniProt Related Accession #

P05783

NCBI Official Full Name

cytokeratin 18

NCBI Official Synonym Full Names

keratin 18

NCBI Official Symbol

KRT18

NCBI Official Synonym Symbols

K18; CK-18; CYK18

UniProt Protein Name

Keratin, type I cytoskeletal 18

UniProt Synonym Protein Names

Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Protein Family

Keratin

UniProt Gene Name

KRT18

UniProt Synonym Gene Names

CYK18; CK-18; K18

NCBI Protein Information

keratin, type I cytoskeletal 18

NCBI Summary for KRT18

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for KRT18

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Research Articles on KRT18

1. Plasma M30-M65 levels are higher in serum of patients with placental abruption.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-KRT18 antibody

Reference Product	PubMed Publications
KRT8 antibody	>68 publications with KRT18 and KRT8
CASP3 antibody	>11 publications with KRT18 and CASP3
CDH1 antibody	>9 publications with KRT18 and CDH1
DSP antibody	>5 publications with KRT18 and DSP
TCHP antibody	>1 publications with KRT18 and TCHP
CASP6 antibody	>1 publications with KRT18 and CASP6

Pathways associated with anti-KRT18 antibody

Products by Pathway	Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Keratinization Pathway antibodies	Keratinization Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with anti-KRT18 antibody

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>66 publications with KRT18 and Liver Diseases
Fatty Liver Antibodies	>30 publications with KRT18 and Fatty Liver
Fibrosis Antibodies	>27 publications with KRT18 and Fibrosis
Neoplasm Metastasis Antibodies	>19 publications with KRT18 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>18 publications with KRT18 and Breast Neoplasms
Inflammation Antibodies	>16 publications with KRT18 and Inflammation
Liver Cirrhosis Antibodies	>16 publications with KRT18 and Liver Cirrhosis
Necrosis Antibodies	>15 publications with KRT18 and Necrosis
Liver Neoplasms Antibodies	>14 publications with KRT18 and Liver Neoplasms
Hepatitis Antibodies	>12 publications with KRT18 and Hepatitis