Mouse Anti-PAX-8 Monoclonal Antibody – Cat# MBS346026

IHC (paraffin) of human thyroid gland using PAX-8 Antibody

PAX-8 Antibody – Mouse Monoclonal

Name: PAX-8 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS346026
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS346026

PAX-8 is a transcription factor in the paired box (PAX) family, whose mainly functions as important regulators in early development, especially in thyroid follicular cells and thyroid specific genes. Mutations in PAX-8 could lead to thyroid follicular… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

PAX-8, Monoclonal Antibody

Full Product Name

PAX-8 Antibody

Product Gene Name

anti-PAX-8 antibody

Clonality

Monoclonal

Isotype

IgG

Clone Number

IHC008

Host

Mouse

Species Reactivity

Human

Form/Format

Liquid

Preparation and Storage

Store at 2-8 degree C. Do not freeze.

Molecular Weight

6,524 Da

3D Structure

ModBase 3D Structure for Q99573

Buffer

Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3

IHC Positive Control

Ovarian, Thyroid, and Renal Cell Carcinomas

IHC Suggested Protocol

1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block – Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)

Applications Tested/Suitable for anti-PAX-8 antibody

Immunohistochemistry (IHC) Paraffin

Application Notes for anti-PAX-8 antibody

Dilution Range: 1:100 - 1:200

Other Notes

Small volumes of anti-PAX-8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PAX-8 purchase

MBS346026 is an antibody targeting PAX-8. Also known as PAX-8 Antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PAX-8. It may not necessarily be applicable to this product.

NCBI GI #

1850829

NCBI GeneID

7849

NCBI Accession #

CAA67903.1

UniProt Primary Accession #

Q99573

UniProt Related Accession #

Q06710

NCBI Official Full Name

Pax-8, partial

NCBI Official Synonym Full Names

paired box 8

NCBI Official Symbol

PAX8

UniProt Protein Name

Pax-8 protein

UniProt Synonym Protein Names

Pax-8 protein

Protein Family

Paired box protein

UniProt Gene Name

Pax-8

NCBI Protein Information

paired box protein Pax-8

NCBI Summary for PAX-8

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

Research Articles on PAX-8

1. PAX8-PPARG fusions may not play major roles in the tumorigenesis of paediatric follicular thyroid carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PAX-8 antibody

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