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Macrophage HAM-56 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS370085

Macrophages comprise many forms of mononuclear phagocytes found in tissues that derive from hematopoietic stem cells in the bone marrow. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, killing of ingested microorganisms, and… More details

$195 / 5 Slides +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Macrophage HAM-56, Monoclonal Antibody
Full Product Name
Macrophage HAM-56
Clonality
Monoclonal
Isotype
IgM/K
Clone Number
HAM-56
Host
Mouse
Species Reactivity
Human, Monkey
Preparation and Storage
Store at 2-8°C (Control slides: Store at 20°C-25°C).
Control
Tonsil, Lymph Node
Localization
Cytoplasmic
Immunogen
Sonicated human alveolar marcrophages.
Intended Use
This antibody is intended for use in Immunohistochemical applications on formalin-fixed paraffin-embedded tissues (FFPE), frozen tissue sections, and cell preparations.
Reactivity Note
Paraffin, Frozen
Precautions
1.This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.
2. Always wear personal protective equipment such as a laboratory coat, goggles, and gloves when handling reagents.
3. Dispose of unused solution with copious amounts of water.
4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.
5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.
6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).
7. For additional safety information refer to Safety Data Sheet for this product.
8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and AMDL".
Mounting Protocols
For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter.
Product Limitations
Due to inherent variability present in immunohistochemical procedures (including fixation time of tissues, dilution factor of antibody, retrieval method utilized, and incubation time), optimal performance should be established through the use of positive and negative controls.
ISO Certification
Manufactured in an ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-Macrophage HAM-56 antibody
Immunohistochemistry (IHC), Leukemia & Histiocytic
Application Notes for anti-Macrophage HAM-56 antibody
Paraffin sections:
The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate , ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions .

Frozen sections and cell preparations:
The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.

IHC Staning Protocol:
1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides
2. Air dry for 2 hours at 58° C.
3. Deparaffinize, dehydrate and rehydrate tissues.
4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate .
5. Any of three heating methods may be used:
a. TintoRetriever r Pressure Cooker or Equivalent
Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.
b. TintoRetriever PT Module or Water Bath Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA
Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.
c. Conventional Steamer Method
Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA
Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.
6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.
7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.
8. Wash slides with IHC wash buffer or DI water.
9. Continue IHC staining protocol

IHC Protocol of anti-Macrophage HAM-56 antibody

Mouse Anti-Macrophage HAM-56 Monoclonal Antibody – Cat# MBS370085

Immunohistochemistry (IHC) of anti-Macrophage HAM-56 antibody

ICH of Macrophage HAM-56 on an FFPE Liver Tissue
Mouse Anti-Macrophage HAM-56 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS370085

Other Notes

Small volumes of anti-Macrophage HAM-56 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Macrophage HAM-56 purchase

MBS370085 is an antibody targeting Macrophage HAM-56. Reactive in Human, Monkey. Host: Mouse.
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