Renal Cell Carcinoma (CA9) Antibody – Mouse Monoclonal

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jul 2008]

Function: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Ref.12

Catalytic activity: H2CO3 = CO2 + H2O.

Cofactor: Zinc.

Enzyme regulation: Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Ref.10 Ref.11 Ref.14 Ref.15

Subunit structure: Forms oligomers linked by disulfide bonds. Ref.12 Ref.17

Subcellular location: Nucleus. Nucleus › nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection › microvillus membrane; Single-pass type I membrane protein. Note: Found on the surface microvilli and in the nucleus, particularly in nucleolus. Ref.8

Tissue specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Induction: By hypoxia. Ref.10 Ref.11 Ref.12 Ref.14 Ref.15

Post-translational modification: Asn-346 bears high-mannose type glycan structures.

Sequence similarities: Belongs to the alpha-carbonic anhydrase family.

Biophysicochemical propertiespH dependence:Optimum pH is 6.5. Ref.17