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ALY Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS375058

MBS375058 is an antibody targeting ALY. Also known as Mouse Anti-ALY [11G5]. Reactive in Mouse, Human, Xenopus laevis. Host: Mouse. More details

$545 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ALY, Monoclonal Antibody
Full Product Name
Mouse Anti-ALY [11G5]
Product Gene Name
Product Synonym Names
Ally of AML1 and LEF1 antibody; BEF antibody; bZIP enhancing factor antibody; REF antibody; THO complex 4antibody; THOC 4 antibody; THOC-4 antibody; THOC4 antibody; Transcriptional coactivatorantibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
11G5
Host
Mouse
Species Reactivity
Mouse, Human, Xenopus laevis
Form/Format
Purified antibody (from supernatant) containing PBS 0.1% sodium azide;
Purity/Purification
Protein A
Concentration
1mg/ml (lot specific)
Target Antigen
ALY
Immunogen
Recombinant human ALY / REF fusion protein
Myeloma/fusion Partners
SP2/0
Research Areas
Transcription
Applications Tested/Suitable for anti-ALY antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Electron Microscopy, ChIP
Application Notes for anti-ALY antibody
Optimal antibody dilution should be determined by titration, however as a guideline try: IHC-P: Use a concentration of 1 ug/ml. ICC/IF: 1/50. PubMed: 17103222 WB: 1/1500. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).

Immunohistochemistry (IHC) of anti-ALY antibody

Mouse Anti-ALY Monoclonal Antibody – Cat# MBS375058

Product References and Citations for anti-ALY antibody

Ding D et al. The role of deimination in ATP5b mRNA transport in a transgenic mouse model of multiple sclerosis. EMBO Rep 13:230-6 (2012). WB, Electron Microscopy ; Mouse PMID: 22261716 Dominguez-Sanchez MS et al. Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human cancers. BMC Cancer 11:77 (2011). IHC-P ; Human PMID: 21329510 Thomas M et al. The Cellular DExD/H-Box RNA-Helicases UAP56 and URH49 Exhibit a CRM1-Independent Nucleocytoplasmic Shuttling Activity. PLoS One 6:e22671 (2011). WB ; Human PMID: 21799930 Read EK & Digard P Individual influenza A virus mRNAs show differential dependence on cellular NXF1/TAP for their nuclear export. J Gen Virol 91:1290-301 (2010). WB, In Situ Hybridisation ; Human PMID: 20071484 Katahira J et al. Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA. EMBO J 28:556-67 (2009). WB ; Human PMID: 19165146 Taniguchi I et al. ATP-dependent recruitment of export factor Aly/REF onto intronless mRNAs by RNA helicase UAP56.Mol Cell Biol. 2008 Jan;28(2):601-8. Epub 2007 Nov 5.IP ; Xenopus laevis PMID: 17984224 van Koningsbruggen S et al. FRG1P-mediated aggregation of proteins involved in pre-mRNA processing. Chromosoma 116:53-64 (2007) PMID: 17103222 Sugiura T et al. Intracellular characterization of DDX39, a novel growth-associated RNA helicase.Exp Cell Res. 2007 Feb 15;313(4):782-90. Epub 2006 Dec 5.WB ; Human PMID: 17196963 Nojima T et al. The interaction between cap-binding complex and RNA export factor is required for intronless mRNA export. J Biol Chem 282:15645-51 (2007) PMID: 17363367 Suganuma H et al. Aly/ REF, a factor for mRNA transport, activates RH gene promoter function.FEBS J. 2005 Jun;272(11):2696-704.ChIP ; Human PMID: 15943804 The Y14 protein communicates to the cytoplasm the position of exonexon junctions. KIM VN (Embo J,Apr 2001)PMID: 11296238

Other Notes

Small volumes of anti-ALY antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ALY. It may not necessarily be applicable to this product.

Protein Family

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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