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hnRNP U Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS375149

MBS375149 is an antibody targeting hnRNP U. Also known as Mouse Anti-hnRNP U [3G6]. Reactive in Mouse, Human, Monkey. Does not react with Chicken, Lizard. Host: Mouse. More details

$545 / 0.05 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
hnRNP U, Monoclonal Antibody
Full Product Name
Mouse Anti-hnRNP U [3G6]
Product Gene Name
Product Synonym Names
Heterogeneous nuclear ribonucleoprotein U antibody; hnRNP U protein antibody; HNRNPU antibody; hnRNPUprotein antibody; HNRPU antibody; p120 antibody; p120 nuclear protein antibody; pp120 antibody; SAF Aantibody; SAF-A antibody; SAFA antibody; Scaffold attachment factor A antibody; U21.1antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3G6
Host
Mouse
Species Reactivity
Mouse, Human, Monkey. Does not react with Chicken, Lizard
Form/Format
Purified antibody (from supernatant) containing PBS 0.1% sodium azide;
Purity/Purification
Protein A
Concentration
1mg/ml (lot specific)
Molecular Weight
88,980 Da
OMIM
602869
3D Structure
ModBase 3D Structure for Q00839
Target Antigen
hnRNP U
Immunogen
RNPs eluted from an oligo (dt) cellulose column
Myeloma/fusion Partners
SP2/0
Research Areas
RNA binding; RNA Processing
Applications Tested/Suitable for anti-hnRNP U antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ChIP
Application Notes for anti-hnRNP U antibody
Optimal antibody dilution should be determined by titration, however as a guideline try; WB: Use at an assay dependent dilution. Detects a band of approximately 97 kDa (predicted molecular weight: 120 kDa). ICC/IFICC/IF: 1/100. Flow CytFlow Cyt: Use 1ug for 106 cells.

Immunohistochemistry (IHC) of anti-hnRNP U antibody

Mouse Anti-hnRNP U Monoclonal Antibody – Cat# MBS375149

Product References and Citations for anti-hnRNP U antibody

Ma N et al. The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles. J Cell Sci 124:394-404 (2011) PUBMED Tserel L et al. MicroRNA Expression Profiles of Human Blood Monocyte-derived Dendritic Cells and Macrophages Reveal miR-511 as Putative Positive Regulator of Toll-like Receptor 4. J Biol Chem 286:26487-95 (2011). WB ; Human PUBMED Raju CS et al. In neurons, activity-dependent association of dendritically transported mRNA transcripts with the transacting factor CBF-A is mediated by A2RE/RTS elements. Mol Biol Cell 22:1864-77 (2011). WB ; Mouse PUBMED Pare JM et al. MicroRNA-binding is required for recruitment of human Argonaute 2 to stress granules and P-bodies. Biochem Biophys Res Commun : (2011). WB ; Human PUBMED Hadian K et al. Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev protein. J Biol Chem 284:33384-91 (2009). WB ; Human PUBMED Pacheco A et al. A novel role for Gemin5 in mRNA translation. Nucleic Acids Res. 2009 Feb;37(2):582-90. doi: 10.1093/nar/gkn979. Epub 2008 Dec 9. WB, IP; HumanPMID: 19066202 Uchimura Y et al. Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. J Biol Chem. 2006 Aug 11;281(32):23180-90. Epub 2006 Jun 5. ICC/IF; HumanPMID: 16757475 HnRNP L represses exon splicing via a regulated exonic splicing silencer. Rothrock CR et al. EMBO J. 2005 Aug 3;24(15):2792-802. Epub 2005 Jul 7PUBMED Ahmad N et al. Kruppel-like factor 2 transcriptional regulation involves heterogeneous nuclear ribonucleoproteins and acetyltransferases. Biochemistry. 2005 Apr 26;44(16):6276-85. ChIP, EMSA; MousePMID: 15835916 Dreyfuss G et al. Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies. Mol Cell Biol 4:1104-14 (1984) PUBMED

Other Notes

Small volumes of anti-hnRNP U antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for hnRNP U. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
heterogeneous nuclear ribonucleoprotein U isoform a
NCBI Official Synonym Full Names
heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
NCBI Official Symbol
HNRNPU
NCBI Official Synonym Symbols
SAFA; HNRPU; SAF-A; U21.1; hnRNP U
UniProt Protein Name
Heterogeneous nuclear ribonucleoprotein U
UniProt Synonym Protein Names
Scaffold attachment factor A; SAF-A; p120; pp120
UniProt Gene Name
HNRNPU
UniProt Synonym Gene Names
HNRPU; SAFA; U21.1; hnRNP U; SAF-A
UniProt Entry Name
HNRPU_HUMAN
NCBI Protein Information
heterogeneous nuclear ribonucleoprotein U; p120 nuclear protein; pp120

NCBI Summary for hnRNP U

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain. This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis, this protein is cleaved in a caspase-dependent way. Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites. But this cleavage does not affect the function of the encoded protein in RNA metabolism. At least two alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for hnRNP U

hnRNP U: Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single- stranded DNA and RNA. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding; Nuclear receptor co-regulator; Spliceosome; DNA-binding

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: nucleoplasm; cell surface; membrane; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; DNA binding; RNA binding; ATP binding

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; osteoblast differentiation; RNA splicing; gene expression; circadian regulation of gene expression
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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