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MCM6 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3900779

The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6,… More details

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Product Overview

Online Datasheet in PDF
Product Name
MCM6, Polyclonal Antibody
Full Product Name
Goat anti-MCM6 Antibody, Affinity Purified
Product Gene Name
anti-MCM6 antibody
Product Synonym Names
minichromosome maintenance deficient (mis5, S. pombe) 6; Mis5; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); MCG40308; DNA replication licensing factor MCM6; P105MCM; DNA replication licensing factor MCM6; minichromosome maintenance complex component 6
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
92,889 Da
OMIM
phenotype 601806
3D Structure
ModBase 3D Structure for Q14566
Predicted Reactivity
Bovine
Immunogen
Between 771 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-MCM6 antibody

Primary Antibodies
Applications Tested/Suitable for anti-MCM6 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-MCM6 antibody
WB: 1:2000-1:10,000
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-MCM6 antibody

Detection of human MCM6 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-MCM6 (Cat. No. MBS3900779 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Goat Anti-MCM6 Polyclonal Antibody – Cat# MBS3900779

Western Blot (WB) of anti-MCM6 antibody

Detection of human MCM6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody MBS3900779 (lot MBS3900779-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Goat Anti-MCM6 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3900779

Other Notes

Small volumes of anti-MCM6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCM6 purchase

MBS3900779 is an antibody targeting MCM6. Also known as Goat anti-MCM6 Antibody, Affinity Purified. Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for MCM6. It may not necessarily be applicable to this product.

NCBI GI #
7427519
NCBI GeneID
4175
NCBI Accession #
NP_005906.2
UniProt Primary Accession #
Q14566
UniProt Secondary Accession #
Q13504; Q99859; B2R6H2
UniProt Related Accession #
Q14566
GenBank Nucleotide #
NM_005915.5
NCBI Official Full Name
DNA replication licensing factor MCM6
NCBI Official Synonym Full Names
minichromosome maintenance complex component 6
NCBI Official Symbol
MCM6
NCBI Official Synonym Symbols
Mis5; P105MCM; MCG40308
UniProt Protein Name
DNA replication licensing factor MCM6
UniProt Synonym Protein Names
p105MCM
Protein Family
DNA replication licensing factor
UniProt Gene Name
MCM6
UniProt Entry Name
MCM6_HUMAN
NCBI Protein Information
DNA replication licensing factor MCM6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); minichromosome maintenance deficient (mis5, S. pombe) 6

NCBI Summary for MCM6

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood. [provided by RefSeq, May 2012]
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UniProt Comments for MCM6

MCM6: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Belongs to the MCM family.

Protein type: EC 3.6.4.12; Cell cycle regulation

Chromosomal Location of Human Ortholog: 2q21

Cellular Component: nucleoplasm; MCM complex; nucleus

Molecular Function: ATP-dependent DNA helicase activity; identical protein binding; protein binding; single-stranded DNA binding; ATP binding

Biological Process: DNA unwinding during replication; DNA replication initiation; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; G1/S transition of mitotic cell cycle

Disease: Lactose Intolerance, Adult Type
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Research Articles on MCM6

1. Mcm2-7 loads onto origins during initiation as a double hexamer, yet does not act as a double-stranded DNA pump during elongation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MCM6 antibody

 Reference Product  PubMed Publications
 MCM2 antibody  >80 publications with MCM6 and MCM2
 MCM7 antibody  >71 publications with MCM6 and MCM7
 MCM4 antibody  >69 publications with MCM6 and MCM4
 MCM3 antibody  >36 publications with MCM6 and MCM3
 MCM5 antibody  >34 publications with MCM6 and MCM5
 CDT1 antibody  >19 publications with MCM6 and CDT1
 CDC45 antibody  >13 publications with MCM6 and CDC45
 GMNN antibody  >3 publications with MCM6 and GMNN
 MCM10 antibody  >3 publications with MCM6 and MCM10

Pathways associated with anti-MCM6 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-MCM6 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >14 publications with MCM6 and Carcinoma
 Lung Neoplasms Antibodies  >8 publications with MCM6 and Lung Neoplasms
 Adenocarcinoma Antibodies  >7 publications with MCM6 and Adenocarcinoma
 Nervous System Diseases Antibodies  >7 publications with MCM6 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >5 publications with MCM6 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >4 publications with MCM6 and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >4 publications with MCM6 and Liver Neoplasms
 Liver Diseases Antibodies  >4 publications with MCM6 and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >3 publications with MCM6 and Cell Transformation, Neoplastic
 Carcinoma, Hepatocellular Antibodies  >2 publications with MCM6 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-MCM6 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >19 publications with MCM6 and Blood
 Lung Antibodies  >10 publications with MCM6 and Lung
 Vascular Antibodies  >9 publications with MCM6 and Vascular
 Lymph Antibodies  >7 publications with MCM6 and Lymph
 Liver Antibodies  >7 publications with MCM6 and Liver
 Muscle Antibodies  >6 publications with MCM6 and Muscle
 Lymph Node Antibodies  >6 publications with MCM6 and Lymph Node
 Eye Antibodies  >4 publications with MCM6 and Eye
 Ovary Antibodies  >4 publications with MCM6 and Ovary
 Bone Antibodies  >4 publications with MCM6 and Bone
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