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QKI Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3900818

QKI is an RNA-binding protein and translational repressor important to embryogenesis and myelination. QKI exist as multiple isoforms with distinct functions that regulate pre-mRNA splicing and the stability of proteins central to oligodendrocyte… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
QKI, Polyclonal Antibody
Full Product Name
Rabbit anti-QKI Antibody, Affinity Purified
Product Gene Name
anti-QKI antibody
Product Synonym Names
QK1; RNA binding protein HQK; quaking homolog, KH domain RNA binding; QKI/LOC100132735 fusion; QK3; protein quaking; hqkI; homolog of mouse quaking QKI (KH domain RNA binding protein); Hqk; QK; Protein quaking; QKI, KH domain containing RNA binding
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
609590
3D Structure
ModBase 3D Structure for Q96PU8
Predicted Reactivity
Rat, Zebrafish, X. laevis, Chicken, Bovine, Dog, Horse, Pig, Cat
Immunogen
Between 291 and C-terminus of isoform 5
Conjugation
Unconjugated

Product Categories/Family for anti-QKI antibody

Primary Antibodies
Applications Tested/Suitable for anti-QKI antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-QKI antibody
WB: 1:5000-1:25,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-QKI antibody

Detection of human and mouse QKI by immunohistochemistry. Sample: FFPE sections of human testicular seminoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-QKI (Cat. No. MBS3900818 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-QKI Polyclonal Antibody – Cat# MBS3900818

Immunoprecipitation (IP) of anti-QKI antibody

Detection of human QKI by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-QKI antibody MBS3900818 (lot MBS3900818-2) used for IP at 6 ug/mg lysate. QKI was also immunoprecipitated by a prior lot (MBS3900818-1) of this antibody. For blotting immunoprecipitated QKI, MBS3900818 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-QKI Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3900818

Western Blot (WB) of anti-QKI antibody

Detection of human and mouse QKI by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (50ug), and mouse NIH 3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-QKI antibody MBS3900818 (lot MBS3900818-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-QKI Polyclonal Antibody, Western Blot (WB) – Cat# MBS3900818

Other Notes

Small volumes of anti-QKI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for QKI purchase

MBS3900818 is an antibody targeting QKI. Also known as Rabbit anti-QKI Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for QKI. It may not necessarily be applicable to this product.

NCBI GeneID
9444
NCBI Accession #
AAH19917.1
UniProt Primary Accession #
Q96PU8
UniProt Protein Name
Protein quaking
Protein Family
Protein quaking
UniProt Gene Name
QKI
UniProt Synonym Gene Names
HKQ; Hqk; HqkI
UniProt Entry Name
QKI_HUMAN

UniProt Comments for QKI

QKI: RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor. 6 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: cytoplasm; nucleus

Molecular Function: mRNA binding; protein binding; SH3 domain binding

Biological Process: myelination; regulation of translation; muscle cell differentiation; mRNA transport; RNA splicing; long-chain fatty acid biosynthetic process; mRNA processing; vasculogenesis; spermatid development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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