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hSET1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3900904

hSET1 is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. The Set class of proteins takes its name from the 130-140 aa motif SET domain… More details

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Product Overview

Online Datasheet in PDF
Product Name
hSET1, Polyclonal Antibody
Full Product Name
Rabbit anti-hSET1 Antibody, Affinity Purified
Product Gene Name
anti-hSET1 antibody
Product Synonym Gene Name
SETD1A
Product Synonym Names
Set1A; histone-lysine N-methyltransferase SETD1A; hSET1A; KMT2F; lysine N-methyltransferase 2F; SET domain-containing protein 1A; Set1; set1/Ash2 histone methyltransferase complex subunit SET1; Histone-lysine N-methyltransferase SETD1A; SET domain containing 1A
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
186,034 Da
Chromosome Location
Chromosome: 16; NC_000016.9 (30968615..30995985). Location: 16p11.2
OMIM
AB002337 mRNA
3D Structure
ModBase 3D Structure for O15047
Immunogen
Between 1200 and 1250
Conjugation
Unconjugated

Product Categories/Family for anti-hSET1 antibody

Primary Antibodies
Applications Tested/Suitable for anti-hSET1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-hSET1 antibody
WB: 1:10,000-1:25,000
IP: 6ug/mg lysate
IHC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Chromatin Immunoprecipitation (ChIP) of anti-hSET1 antibody

Chip-chip scatter plot of anti-hSET1 (MBS3900904) enriched DNA binding sites versus input reference DNA. A. 10 ug of MBS3900904 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-hSET1 ChIP, normal rabbit IgG showed little enrichment.
Rabbit Anti-hSET1 Polyclonal Antibody – Cat# MBS3900904

Immunohistochemistry (IHC) of anti-hSET1 antibody

Detection of human hSET1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-hSET1 (Cat. No. MBS3900904 Lot 8) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-hSET1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3900904

Immunoprecipitation (IP) of anti-hSET1 antibody

Detection of human hSET1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-hSET1 antibody (MBS3900904 lot 8) used for IP at 6 ug per reaction. hSET1 was also immunoprecipitated by a previous lot of this antibody (MBS3900904 lot 7). For blotting immunoprecipitated hSET1, MBS3900904 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Rabbit Anti-hSET1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3900904

Western Blot (WB) of anti-hSET1 antibody

Detection of human hSET1 by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, Hep-G2, MCF-7, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-hSET1 antibody (MBS3900904 lot 8) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-hSET1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3900904

Western Blot (WB) of anti-hSET1 antibody

Detection of mouse hSET1 by western blot. Samples: Whole cell lysate (25 ug) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-hSET1 antibody (MBS3900904 lot 8) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-hSET1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3900904

Other Notes

Small volumes of anti-hSET1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for hSET1 purchase

MBS3900904 is an antibody targeting hSET1. Also known as Rabbit anti-hSET1 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for hSET1. It may not necessarily be applicable to this product.

NCBI GI #
55741677
NCBI GeneID
9739
NCBI Accession #
NP_055527.1
UniProt Primary Accession #
O15047
UniProt Secondary Accession #
Q6PIF3; Q8TAJ6; A6NP62
UniProt Related Accession #
O15047
GenBank Nucleotide #
NM_014712.1
NCBI Official Full Name
histone-lysine N-methyltransferase SETD1A
NCBI Official Synonym Full Names
SET domain containing 1A
NCBI Official Symbol
SETD1A
NCBI Official Synonym Symbols
Set1; KMT2F; Set1A
UniProt Protein Name
Histone-lysine N-methyltransferase SETD1A
UniProt Synonym Protein Names
Lysine N-methyltransferase 2F; SET domain-containing protein 1A; hSET1A; Set1/Ash2 histone methyltransferase complex subunit SET1
UniProt Gene Name
SETD1A
UniProt Synonym Gene Names
; hSET1A
NCBI Protein Information
histone-lysine N-methyltransferase SETD1A; hSET1A; lysine N-methyltransferase 2F; SET domain-containing protein 1A; set1/Ash2 histone methyltransferase complex subunit SET1

UniProt Comments for hSET1

SETD1A: Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys- 9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.

Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: histone methyltransferase complex; nuclear chromatin; nuclear speck; nucleoplasm; nucleus

Molecular Function: beta-catenin binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; protein binding; transcription factor binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-hSET1 antibody

 Reference Product  PubMed Publications
 ASH2L antibody  >6 publications with hSET1 and ASH2L
 RBBP5 antibody  >5 publications with hSET1 and RBBP5
 WDR5 antibody  >5 publications with hSET1 and WDR5
 WDR82 antibody  >4 publications with hSET1 and WDR82
 CXXC1 antibody  >4 publications with hSET1 and CXXC1
 HCFC1 antibody  >2 publications with hSET1 and HCFC1
 DPY30 antibody  >1 publications with hSET1 and DPY30

Pathways associated with anti-hSET1 antibody

 Products by Pathway  Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram
 Lysine Degradation Pathway antibodies  Lysine Degradation Pathway Diagram

Diseases associated with anti-hSET1 antibody

 Disease Name  Pubmed Publications
 Anemia Antibodies  >1 publications with hSET1 and Anemia
 Necrosis Antibodies  >1 publications with hSET1 and Necrosis

Organs/Tissues associated with anti-hSET1 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >2 publications with hSET1 and Kidney
 Bone Antibodies  >1 publications with hSET1 and Bone
 Connective Tissue Antibodies  >1 publications with hSET1 and Connective Tissue
 Blood Antibodies  >1 publications with hSET1 and Blood
 Muscle Antibodies  >1 publications with hSET1 and Muscle
 Lung Antibodies  >1 publications with hSET1 and Lung
 Testis Antibodies  >1 publications with hSET1 and Testis
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