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anti-LYN antibody :: Goat LYN Polyclonal Antibody

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Catalog # MBS420888
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

LYN, Polyclonal Antibody

Full Product Name

Goat anti-LYN Antibody

Product Synonym Names
LYN; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog; FLJ26625; JTK8; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog; LYN antibody; v-yes-1 Yamaguchi sarcoma viral related oncogene homolog antibody; FLJ26625 antibody; JTK8 antibody; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog antibody
Product Gene Name
Antibody/Peptide Pairs
LYN peptide (MBS425011) is used for blocking the activity of LYN antibody (MBS420888)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
KTQPVRNTER T
OMIM
165120
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-KTQPVRNTERT, from the internal region of the protein sequence according to NP_002341.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-LYN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-LYN antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-LYN antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 60kDa band observed in lysates of cell line U937 (calculated MW of 58.6kDa according to NP_002341.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-LYN antibody
(0.1ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-LYN antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for LYN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,033 Da
NCBI Official Full Name
tyrosine-protein kinase Lyn isoform A
NCBI Official Synonym Full Names
LYN proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
JTK8; p53Lyn; p56Lyn
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NCBI Protein Information
tyrosine-protein kinase Lyn
UniProt Protein Name
Tyrosine-protein kinase Lyn
UniProt Synonym Protein Names
Lck/Yes-related novel protein tyrosine kinase; V-yes-1 Yamaguchi sarcoma viral related oncogene homolog; p53Lyn; p56Lyn
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
LYN_HUMAN
NCBI Summary for LYN
This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
UniProt Comments for LYN
LYN: a tyrosine kinase of the Src family that plays a role in B cell antigen-receptor signaling.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Oncoprotein; Kinase, protein; TK group; Src family

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; Golgi apparatus; lipid raft; mast cell granule; mitochondrial crista; mitochondrial intermembrane space; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic density

Molecular Function: ATP binding; enzyme binding; gamma-tubulin binding; glycosphingolipid binding; integrin binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; platelet-derived growth factor receptor binding; protein binding; protein-tyrosine kinase activity; receptor binding; receptor signaling protein tyrosine kinase activity; SH3 domain binding; ubiquitin protein ligase binding

Biological Process: adaptive immune response; B cell homeostasis; B cell receptor signaling pathway; blood coagulation; cellular response to extracellular stimulus; central nervous system development; cytokine secretion; ephrin receptor signaling pathway; erythrocyte differentiation; Fc receptor mediated inhibitory signaling pathway; Fc receptor mediated stimulatory signaling pathway; histamine secretion by mast cell; immune response-regulating cell surface receptor signaling pathway; inflammatory response; innate immune response; leukocyte migration; lipopolysaccharide-mediated signaling pathway; negative regulation of B cell proliferation; negative regulation of cell proliferation; negative regulation of immune response; negative regulation of MAP kinase activity; negative regulation of myeloid leukocyte differentiation; negative regulation of protein amino acid phosphorylation; negative regulation of toll-like receptor 2 signaling pathway; negative regulation of toll-like receptor 4 signaling pathway; neurite development; oligodendrocyte development; peptidyl-tyrosine phosphorylation; platelet activation; platelet degranulation; positive regulation of B cell receptor signaling pathway; positive regulation of cell migration; positive regulation of cell motility; positive regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of tyrosine phosphorylation of STAT protein; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of B cell apoptosis; regulation of B cell receptor signaling pathway; regulation of cell adhesion mediated by integrin; regulation of cytokine production; regulation of cytokine secretion; regulation of erythrocyte differentiation; regulation of inflammatory response; regulation of mast cell activation; regulation of mast cell degranulation; regulation of protein amino acid phosphorylation; regulation of release of sequestered calcium ion into cytosol; response to amino acid stimulus; response to axon injury; response to carbohydrate stimulus; response to DNA damage stimulus; response to drug; response to hormone stimulus; response to insulin stimulus; response to organic cyclic substance; response to sterol depletion; response to toxin; signal transduction; stimulatory C-type lectin receptor signaling pathway; T cell costimulation; tolerance induction to self antigen; transmembrane receptor protein tyrosine kinase signaling pathway; viral reproduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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