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FUS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3900916

FUS has been identified as a frequent translocation fusion partner of various transcription factors. FUS fusion genes have been shown to be associated with multiple tumor types which include liposarcoma, leukemia, histocytoma, and sarcoma. FUS is a… More details

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Product Overview

Online Datasheet in PDF
Product Name
FUS, Polyclonal Antibody
Full Product Name
Rabbit anti-FUS Antibody, Affinity Purified
Product Gene Name
anti-FUS antibody
Product Synonym Names
TLS; HNRNPP2; oncogene FUS; translocated in liposarcoma protein; oncogene TLS; FUS/ERG chimeric protein; RNA-binding protein FUS; heterogeneous nuclear ribonucleoprotein P2; POMP75; fusion gene in myxoid liposarcoma; fused in sarcoma; FUS/ERG fusion protein; ETM4; ALS6; 75 kDa DNA-pairing protein; fus-like protein; FUS1; RNA-binding protein FUS; FUS RNA binding protein
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
53,355 Da
OMIM
137070
3D Structure
ModBase 3D Structure for P35637
Predicted Reactivity
Bovine
Immunogen
Between 1 and 50
Conjugation
Unconjugated

Product Categories/Family for anti-FUS antibody

Primary Antibodies
Applications Tested/Suitable for anti-FUS antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-FUS antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-FUS antibody

Detection of mouse FUS by immunohistochemistry. Sample: FFPE section of mouse renal cells carcinoma. Antibody: Affinity purified rabbit anti-FUS (Cat. No. MBS3900916 Lot4) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-FUS Polyclonal Antibody – Cat# MBS3900916

Immunohistochemistry (IHC) of anti-FUS antibody

Detection of human FUS by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS (Cat. No. MBS3900916 Lot4) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-FUS Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3900916

Immunoprecipitation (IP) of anti-FUS antibody

Detection of human FUS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FUS antibody MBS3900916 (lot MBS3900908-4) used for IP at 3 ug per reaction. FUS was also immunoprecipitated by rabbit anti-FUS recombinant monoclonal antibody (MBS3906250) and rabbit anti-FUS antibody MBS3900908. For blotting immunoprecipitated FUS, MBS3906250 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.
Rabbit Anti-FUS Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3900916

Western Blot (WB) of anti-FUS antibody

Detection of human and mouse FUS by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FUS antibody MBS3900916 (lot MBS3900916-4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-FUS Polyclonal Antibody, Western Blot (WB) – Cat# MBS3900916

Other Notes

Small volumes of anti-FUS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FUS purchase

MBS3900916 is an antibody targeting FUS. Also known as Rabbit anti-FUS Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FUS. It may not necessarily be applicable to this product.

NCBI GI #
4826734
NCBI GeneID
2521
NCBI Accession #
NP_004951.1
UniProt Primary Accession #
P35637
UniProt Secondary Accession #
Q9H4A8
UniProt Related Accession #
P35637
GenBank Nucleotide #
NM_004960.3
NCBI Official Full Name
RNA-binding protein FUS isoform 1
NCBI Official Synonym Full Names
FUS RNA binding protein
NCBI Official Symbol
FUS
NCBI Official Synonym Symbols
TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2
UniProt Protein Name
RNA-binding protein FUS
UniProt Synonym Protein Names
75 kDa DNA-pairing protein; Oncogene FUS; Oncogene TLS; POMp75; Translocated in liposarcoma protein
Protein Family
RNA-binding protein
UniProt Gene Name
FUS
UniProt Synonym Gene Names
TLS
UniProt Entry Name
FUS_HUMAN
NCBI Protein Information
RNA-binding protein FUS

NCBI Summary for FUS

This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]
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UniProt Comments for FUS

hnRNP P2: Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single- stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity. Component of nuclear riboprotein complexes. Interacts with ILF3, TDRD3 and SF1. Interacts through its C-terminus with SFRS13A. Interacts with OTUB1 and SARNP. Ubiquitous. Belongs to the RRM TET family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding; Nuclear receptor co-regulator; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression

Disease: Amyotrophic Lateral Sclerosis 6, With Or Without Frontotemporal Dementia; Tremor, Hereditary Essential, 4
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Research Articles on FUS

1. Depletion of SAFB1 reduced FUS's localization to chromatin-bound fraction and splicing activity, suggesting SAFB1 could tether FUS to chromatin compartment thorough N-terminal DNA-binding motif. Moreover, FUS interacts with another nuclear matrix-associated protein, Matrin3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FUS antibody

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram
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