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JLP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3900940

JLP (JNK-interacting leucine zipper protein) has been identified as a scaffolding protein that functions to position mitogen-activated protein kinases (MAPKs) into specific signaling modules so that successive phosphorylation events of the MAPK pathway can… More details

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Product Overview

Online Datasheet in PDF
Product Name
JLP, Polyclonal Antibody
Full Product Name
Rabbit anti-JLP Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
SPAG9
Product Synonym Names
Sperm-associated antigen 9; Max-binding protein; mitogen-activated protein kinase 8-interacting protein 4; PHET; PIG6; proliferation-inducing gene 6; Proliferation-inducing protein 6; protein highly expressed in testis; sperm surface protein; lung cancer oncogene 4; Sperm-specific protein; sunday driver 1; c-Jun NH2-terminal kinase-associated leucine zipper protein; sperm associated antigen 9 variant 5; HLC-6; cancer/testis antigen 89; C-Jun-amino-terminal kinase-interacting protein 4; CT89; HLC6; JNK-interacting protein 4; human lung cancer oncogene 6 protein; JIP4; JIP-4; JLP; JNK interacting protein; JNK/SAPK-associated protein; JNK-associated leucine-zipper protein; HLC4; C-Jun-amino-terminal kinase-interacting protein 4; sperm associated antigen 9
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
128,607 Da
3D Structure
ModBase 3D Structure for O60271
Immunogen
Between 775 and 825
Conjugation
Unconjugated

Product Categories/Family for anti-JLP antibody

Applications Tested/Suitable for anti-JLP antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-JLP antibody
WB: 1:1000-1:10,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-JLP antibody

Detection of human JLP by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-JLP (Cat. No. MBS3900940 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-JLP Polyclonal Antibody – Cat# MBS3900940

Immunoprecipitation (IP) of anti-JLP antibody

Detection of human JLP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JLP antibody MBS3900940 (lot MBS3900940-2) used for IP at 6 ug/mg lysate. JLP was also immunoprecipitated by a previous lot (MBS3900940-1) of this antibody. For blotting immunoprecipitated JLP, MBS3900940 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-JLP Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3900940

Western Blot (WB) of anti-JLP antibody

Detection of human JLP by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug) and HEK293T (50ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody MBS3900940 (lot MBS3900940-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-JLP Polyclonal Antibody, Western Blot (WB) – Cat# MBS3900940

Western Blot (WB) of anti-JLP antibody

Detection of mouse JLP by western blot. Samples: Whole cell lysate from TCKM (50ug), Renca (50ug), and CT26 (50ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody MBS3900940 (lot MBS3900940-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-JLP Polyclonal Antibody, Western Blot (WB) – Cat# MBS3900940

Other Notes

Small volumes of anti-JLP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for JLP purchase

MBS3900940 is an antibody targeting JLP. Also known as Rabbit anti-JLP Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for JLP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
C-Jun-amino-terminal kinase-interacting protein 4 isoform 3
NCBI Official Synonym Full Names
sperm associated antigen 9
NCBI Official Symbol
SPAG9
NCBI Official Synonym Symbols
JLP; CT89; HLC4; HLC6; JIP4; PHET; PIG6; HLC-6; JIP-4
UniProt Protein Name
C-Jun-amino-terminal kinase-interacting protein 4
UniProt Synonym Protein Names
Cancer/testis antigen 89; CT89; Human lung cancer oncogene 6 protein; HLC-6; JNK-associated leucine-zipper protein; JLP; Mitogen-activated protein kinase 8-interacting protein 4; Proliferation-inducing protein 6; Protein highly expressed in testis; PHET; Sperm surface protein; Sperm-associated antigen 9; Sperm-specific protein; Sunday driver 1
UniProt Gene Name
SPAG9
UniProt Synonym Gene Names
HSS; KIAA0516; MAPK8IP4; SYD1; JIP-4; JNK-interacting protein 4; CT89; HLC-6; JLP; PHET
NCBI Protein Information
C-Jun-amino-terminal kinase-interacting protein 4; sunday driver 1; Max-binding protein; sperm surface protein; lung cancer oncogene 4; JNK interacting protein; cancer/testis antigen 89; JNK/SAPK-associated protein; proliferation-inducing gene 6; protein

UniProt Comments for JLP

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Isoform 5 may play a role in spermatozoa-egg-interaction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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