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Dyskerin (DKC1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9600383

Description: Seems to be involved in the function of telomerase.Function: Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes… More details

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Product Overview

Online Datasheet in PDF
Product Name
Dyskerin (DKC1), Polyclonal Antibody
Full Product Name
Dyskerin Antibody
Product Gene Name
anti-DKC1 antibody
Product Synonym Names
CBF5; CBF5 homolog; Cbf5p homolog; DKC 1; DKC; Dkc1; DKC1_HUMAN; DKCX; Dyskeratosis congenita 1; Dyskeratosis congenita 1 dyskerin; Dyskerin; H/ACA ribonucleoprotein complex subunit 4; NAP 57; NAP57; NOLA 4; NOLA4; Nopp140 associated protein of 57 kDa; Nopp140-associated protein of 57 kDa; Nucleolar protein family A member 4; Nucleolar protein NAP57; snoRNP protein DKC1; XAP 101; XAP101
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
Dyskerin antibody detects endogenous levels of Dyskerin
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
DKC1 peptide (MBS9614936) is used for blocking the activity of DKC1 antibody (MBS9600383)
Molecular Weight
Observed: 57 kDa
Predicted: 58 kDa
3D Structure
ModBase 3D Structure for O60832
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Dyskerin
Subcellular Location
Cytoplasm and Nucleus > Nucleolus. Nucleus > Cajal body. Also localized to cajal bodies.
Tissue Specificity
Ubiquitously expressed.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (91%), Zebrafish (91%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (82%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-DKC1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-DKC1 antibody
WB: 1:500-1:2000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-DKC1 antibody

MBS9600383 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-DKC1 Polyclonal Antibody – Cat# MBS9600383

Western Blot (WB) of anti-DKC1 antibody

Western blot analysis on HeLa cell lysate using Dyskerin Antibody, The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-DKC1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9600383

Immunofluorescence (IF) of anti-DKC1 antibody

MBS9600383 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-DKC1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9600383

Other Notes

Small volumes of anti-DKC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DKC1 purchase

MBS9600383 is an antibody targeting DKC1. Also known as Dyskerin Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DKC1. It may not necessarily be applicable to this product.

NCBI GI #
215599015
NCBI GeneID
1736
NCBI Accession #
NP_001135935.1
UniProt Primary Accession #
O60832
UniProt Secondary Accession #
O43845; Q96G67; Q9Y505; F5BSB3
UniProt Related Accession #
O60832
GenBank Nucleotide #
NM_001142463.2
NCBI Official Full Name
H/ACA ribonucleoprotein complex subunit DKC1 isoform 2
NCBI Official Synonym Full Names
dyskerin pseudouridine synthase 1
NCBI Official Symbol
DKC1
NCBI Official Synonym Symbols
DKC; CBF5; DKCX; NAP57; NOLA4; XAP101
UniProt Protein Name
H/ACA ribonucleoprotein complex subunit DKC1
UniProt Synonym Protein Names
CBF5 homolog; Dyskerin; Nopp140-associated protein of 57 kDa; Nucleolar protein NAP57; Nucleolar protein family A member 4; snoRNP protein DKC1
Protein Family
H/ACA ribonucleoprotein complex
UniProt Gene Name
DKC1
NCBI Protein Information
H/ACA ribonucleoprotein complex subunit DKC1

NCBI Summary for DKC1

This gene functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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UniProt Comments for DKC1

Isoform 1: Catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA (PubMed:25219674). This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1 (PubMed:25219674). Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Required for ribosome biogenesis and telomere maintenance (PubMed:19179534, PubMed:25219674). Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme (PubMed:19179534).
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Research Articles on DKC1

1. The expression of DKC1 was upregulated in ccRCC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DKC1 antibody

 Reference Product  PubMed Publications
 TERT antibody  >55 publications with DKC1 and TERT
 NOP10 antibody  >29 publications with DKC1 and NOP10
 NHP2 antibody  >23 publications with DKC1 and NHP2
 GAR1 antibody  >10 publications with DKC1 and GAR1
 WRAP53 antibody  >2 publications with DKC1 and WRAP53
 RUVBL2 antibody  >2 publications with DKC1 and RUVBL2
 RUVBL1 antibody  >2 publications with DKC1 and RUVBL1
 NOP58 antibody  >1 publications with DKC1 and NOP58

Pathways associated with anti-DKC1 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 Extension Of Telomeres Pathway antibodies  Extension Of Telomeres Pathway Diagram
 H/ACA Ribonucleoprotein Complex Pathway antibodies  H/ACA Ribonucleoprotein Complex Pathway Diagram
 H/ACA Ribonucleoprotein Complex Pathway antibodies  H/ACA Ribonucleoprotein Complex Pathway Diagram
 Regulation Of Telomerase Pathway antibodies  Regulation Of Telomerase Pathway Diagram
 Ribosome Biogenesis In Eukaryotes Pathway antibodies  Ribosome Biogenesis In Eukaryotes Pathway Diagram
 Ribosome Biogenesis In Eukaryotes Pathway antibodies  Ribosome Biogenesis In Eukaryotes Pathway Diagram
 Telomere Extension By Telomerase Pathway antibodies  Telomere Extension By Telomerase Pathway Diagram
 Telomere Maintenance Pathway antibodies  Telomere Maintenance Pathway Diagram

Diseases associated with anti-DKC1 antibody

 Disease Name  Pubmed Publications
 Dyskeratosis Congenita Antibodies  >158 publications with DKC1 and Dyskeratosis Congenita
 Congenital Abnormalities Antibodies  >139 publications with DKC1 and Congenital Abnormalities
 Anemia Antibodies  >36 publications with DKC1 and Anemia
 Fibrosis Antibodies  >15 publications with DKC1 and Fibrosis
 Nervous System Diseases Antibodies  >13 publications with DKC1 and Nervous System Diseases
 Carcinoma Antibodies  >9 publications with DKC1 and Carcinoma
 Disease Models, Animal Antibodies  >7 publications with DKC1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >6 publications with DKC1 and Breast Neoplasms
 Immune System Diseases Antibodies  >5 publications with DKC1 and Immune System Diseases
 Precancerous Conditions Antibodies  >4 publications with DKC1 and Precancerous Conditions

Organs/Tissues associated with anti-DKC1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >88 publications with DKC1 and Bone
 Bone Marrow Antibodies  >87 publications with DKC1 and Bone Marrow
 Blood Antibodies  >40 publications with DKC1 and Blood
 Skin Antibodies  >34 publications with DKC1 and Skin
 Lung Antibodies  >15 publications with DKC1 and Lung
 Brain Antibodies  >10 publications with DKC1 and Brain
 Liver Antibodies  >10 publications with DKC1 and Liver
 Embryonic Tissue Antibodies  >5 publications with DKC1 and Embryonic Tissue
 Prostate Antibodies  >4 publications with DKC1 and Prostate
 Eye Antibodies  >3 publications with DKC1 and Eye
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