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SPTBN1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901442

Beta spectrin (SPTBN1) is a subunit of the spectrin cytoskeletal scaffolding protein that links the plasma membrane to cytoskeletal actin and functions in cellular shape and organelle organization. More details

$250 / 0.002 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
SPTBN1, Polyclonal Antibody
Full Product Name
Rabbit anti-SPTBN1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
beta-II spectrin; epididymis luminal protein 102; spectrin, non-erythroid beta chain 1; spectrin beta chain, non-erythrocytic 1; spectrin beta chain, brain 1; SPTB2; HEL102; fodrin beta chain; embryonic liver beta-fodrin; ELF; betaSpII; beta-spectrin non-erythrocytic 1; beta-spectrin 2; beta-G spectrin; beta-fodrin; beta-spectrin II; Spectrin beta chain, non-erythrocytic 1; spectrin beta, non-erythrocytic 1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
200ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
Observed MW:275kDa
Calculated MW:251kDa/253kDa/274kDa
Chromosome Location
Chromosome: 2; NC_000002.11 (54683454..54898583). Location: 2p21
OMIM
182790
3D Structure
ModBase 3D Structure for Q01082
Immunogen
Between 2125 and 2175
Conjugation
Unconjugated

Product Categories/Family for anti-SPTBN1 antibody

Applications Tested/Suitable for anti-SPTBN1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-SPTBN1 antibody
WB: 1:2000-1:10,000 (3-8% Tris-acetate gel recommended)
IP: 6ug/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-SPTBN1 antibody

Detection of human SPTBN1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SPTBN1 (MBS3901442 Lot 3) used at a dilution of 1:200 (1 ug/ml). Detection: DAB
Rabbit Anti-SPTBN1 Polyclonal Antibody – Cat# MBS3901442

Immunoprecipitation (IP) of anti-SPTBN1 antibody

Detection of human SPTBN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPTBN1 antibody (MBS3901442 lot 3) used for IP at 6 ug per reaction. SPTBN1 was also immunoprecipitated by a previous lot of this antibody (MBS3901442 lot 2) and a second antibody against a different epitope of SPTBN1 (MBS3901443). For blotting immunoprecipitated SPTBN1, MBS3901442 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Rabbit Anti-SPTBN1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3901442

Western Blot (WB) of anti-SPTBN1 antibody

Detection of human SPTBN1 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, HEK293T, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody (MBS3901442 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-SPTBN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901442

Western Blot (WB) of anti-SPTBN1 antibody

Detection of mouse SPTBN1 by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, NIH 3T3, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody (MBS3901442 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Rabbit Anti-SPTBN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901442

Other Notes

Small volumes of anti-SPTBN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SPTBN1 purchase

MBS3901442 is an antibody targeting SPTBN1. Also known as Rabbit anti-SPTBN1 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SPTBN1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
spectrin beta chain, non-erythrocytic 1 isoform 1
NCBI Official Synonym Full Names
spectrin, beta, non-erythrocytic 1
NCBI Official Symbol
SPTBN1
NCBI Official Synonym Symbols
ELF; SPTB2; betaSpII
UniProt Protein Name
Spectrin beta chain, non-erythrocytic 1
UniProt Synonym Protein Names
Beta-II spectrin; Fodrin beta chain; Spectrin, non-erythroid beta chain 1
Protein Family
UniProt Gene Name
SPTBN1
UniProt Synonym Gene Names
SPTB2
UniProt Entry Name
SPTB2_HUMAN
NCBI Protein Information
spectrin beta chain, non-erythrocytic 1; beta-fodrin; beta-G spectrin; beta-spectrin 2; beta-II spectrin; beta-spectrin II; fodrin beta chain; embryonic liver beta-fodrin; spectrin beta chain, brain 1; beta-spectrin non-erythrocytic 1; spectrin, non-eryth

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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