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USP36/DUB1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901445

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can… More details

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Product Overview

Online Datasheet in PDF
Product Name
USP36/DUB1, Polyclonal Antibody
Full Product Name
Rabbit anti-USP36/DUB1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
USP36
Product Synonym Names
ubiquitin specific protease 36; ubiquitin-specific-processing protease 36; ubiquitin thioesterase 36; ubiquitin carboxyl-terminal hydrolase 36; deubiquitinating enzyme 36; DUB1; Ubiquitin carboxyl-terminal hydrolase 36; ubiquitin specific peptidase 36
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
OMIM
612543
3D Structure
ModBase 3D Structure for Q9P275
Immunogen
Between 1073 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-USP36/DUB1 antibody

Applications Tested/Suitable for anti-USP36/DUB1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-USP36/DUB1 antibody
WB: 1:1000-1:5000
IP: 6ug/mg lysate
IHC: 1:2000-1:10,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-USP36/DUB1 antibody

Detection of human USP36/DUB1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-USP36/DUB1(Cat. No. MBS3901445 Lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Rabbit Anti-USP36/DUB1 Polyclonal Antibody – Cat# MBS3901445

Immunoprecipitation (IP) of anti-USP36/DUB1 antibody

Detection of human USP36/DUB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP36/DUB1 antibody MBS3901445 (lot MBS3901445-2) used for IP at 6 ug per reaction. USP36/DUB1 was also immunoprecipitated by rabbit anti-USP36/DUB1 antibody BL4146. For blotting immunoprecipitated USP36/DUB1, MBS3901445 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-USP36/DUB1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3901445

Western Blot (WB) of anti-USP36/DUB1 antibody

Detection of human USP36/DUB1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP36/DUB1 antibody MBS3901445 (lot MBS3901445-2) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Rabbit Anti-USP36/DUB1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901445

Other Notes

Small volumes of anti-USP36/DUB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for USP36/DUB1 purchase

MBS3901445 is an antibody targeting USP36/DUB1. Also known as Rabbit anti-USP36/DUB1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for USP36/DUB1. It may not necessarily be applicable to this product.

NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 36
UniProt Synonym Protein Names
Deubiquitinating enzyme 36; Ubiquitin thioesterase 36; Ubiquitin-specific-processing protease 36
UniProt Gene Name
USP36
UniProt Synonym Gene Names
KIAA1453
UniProt Entry Name
UBP36_HUMAN

UniProt Comments for USP36/DUB1

USP36: May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.19.12; Nucleolus; Ubiquitin-specific protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleolus; nucleus

Molecular Function: cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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