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TRIM3/BERP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901720

TRIM3/BERP is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM3/BERP has been identified as a component of the CART (cytoskeleton-associated recycling or… More details

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Product Overview

Online Datasheet in PDF
Product Name
TRIM3/BERP, Polyclonal Antibody
Full Product Name
Rabbit anti-TRIM3/BERP Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
TRIM3
Product Synonym Names
BERP; RNF22; tripartite motif protein TRIM3; tripartite motif-containing protein 3; RNF97; ring finger protein 22; HAC1; brain expressed ring finger; RING finger protein 97; brain-expressed RING finger protein; Tripartite motif-containing protein 3; tripartite motif containing 3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
67,726 Da
OMIM
605493
3D Structure
ModBase 3D Structure for O75382
Predicted Reactivity
Rat
Immunogen
Between 75 and 125
Conjugation
Unconjugated

Product Categories/Family for anti-TRIM3/BERP antibody

Applications Tested/Suitable for anti-TRIM3/BERP antibody
Immunoprecipitation (IP)
Application Notes for anti-TRIM3/BERP antibody
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-TRIM3/BERP antibody

Detection of human TRIM3/BERP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRIM3/BERP antibody MBS3901720 used for IP at 3 ug/mg lysate. TRIM3/BERP was also immunoprecipitated by rabbit anti-TRIM3/BERP antibodies MBS3901719 and MBS3901721, which recognize other epitopes. For blotting immunoprecipitated TRIM3/BERP, MBS3901721 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-TRIM3/BERP Polyclonal Antibody – Cat# MBS3901720

Other Notes

Small volumes of anti-TRIM3/BERP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRIM3/BERP purchase

MBS3901720 is an antibody targeting TRIM3/BERP. Also known as Rabbit anti-TRIM3/BERP Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIM3/BERP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tripartite motif-containing protein 3 isoform 1
NCBI Official Synonym Full Names
tripartite motif containing 3
NCBI Official Symbol
TRIM3
NCBI Official Synonym Symbols
BERP; HAC1; RNF22; RNF97
UniProt Protein Name
Tripartite motif-containing protein 3
UniProt Synonym Protein Names
Brain-expressed RING finger protein; RING finger protein 22; RING finger protein 97
UniProt Gene Name
TRIM3
UniProt Synonym Gene Names
BERP; RNF22; RNF97
UniProt Entry Name
TRIM3_HUMAN
NCBI Protein Information
tripartite motif-containing protein 3; RING finger protein 97; ring finger protein 22; brain expressed ring finger; tripartite motif-containing 3; tripartite motif protein TRIM3; brain-expressed RING finger protein

NCBI Summary for TRIM3/BERP

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]
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UniProt Comments for TRIM3/BERP

TRIM3: Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Belongs to the TRIM/RBCC family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Cytoskeletal

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; early endosome

Molecular Function: protein C-terminus binding; protein binding; zinc ion binding; ubiquitin-protein ligase activity

Biological Process: nervous system development; protein transport; protein ubiquitination
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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