• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

CHD3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901726

CHD3 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and… More details

$250 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CHD3, Polyclonal Antibody
Full Product Name
Rabbit anti-CHD3 Antibody, Affinity Purified
Product Gene Name
anti-CHD3 antibody
Product Synonym Names
Mi-2a; zinc finger helicase; zinc-finger helicase (Snf2-like); ZFH; Mi2-ALPHA; hZFH; chromodomain-helicase-DNA-binding protein 3; ATP-dependent helicase CHD3; CHD-3; mi-2 autoantigen 240 kDa protein; Chromodomain-helicase-DNA-binding protein 3; chromodomain helicase DNA binding protein 3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
233,037 Da
OMIM
602120
3D Structure
ModBase 3D Structure for Q12873
Immunogen
Between 1600 and 1650
Conjugation
Unconjugated

Product Categories/Family for anti-CHD3 antibody

Primary Antibodies
Applications Tested/Suitable for anti-CHD3 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-CHD3 antibody
WB: 1:2000-1:10,000 (3-8% Tris-acetate gel recommended)
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-CHD3 antibody

Detection of human CHD3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CHD3 (Cat. No. MBS3901726) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-CHD3 Polyclonal Antibody – Cat# MBS3901726

Western Blot (WB) of anti-CHD3 antibody

Detection of human CHD3 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD3 antibody MBS3901726 (lot MBS3901726-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. CHD3 was also immunoprecipitated by a previous lot (lot MBS3901726-1) of this antibody and by rabbit anti-CHD3 antibody MBS3901727, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Rabbit Anti-CHD3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901726

Other Notes

Small volumes of anti-CHD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHD3 purchase

MBS3901726 is an antibody targeting CHD3. Also known as Rabbit anti-CHD3 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CHD3. It may not necessarily be applicable to this product.

NCBI GI #
158420733
NCBI GeneID
1107
NCBI Accession #
NP_001005273.1
UniProt Primary Accession #
Q12873
UniProt Secondary Accession #
Q9Y4I0; D3DTQ9; E9PG89
UniProt Related Accession #
Q12873
GenBank Nucleotide #
NM_001005273
NCBI Official Full Name
Homo sapiens chromodomain helicase DNA binding protein 3 (CHD3), transcript variant 1, mRNA
NCBI Official Synonym Full Names
chromodomain helicase DNA binding protein 3
NCBI Official Symbol
CHD3
NCBI Official Synonym Symbols
ZFH; Mi-2a; Mi2-ALPHA
UniProt Protein Name
Chromodomain-helicase-DNA-binding protein 3
UniProt Synonym Protein Names
ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase
Protein Family
Chromodomain-helicase-DNA-binding protein
UniProt Gene Name
CHD3
UniProt Synonym Gene Names
CHD-3; hZFH
UniProt Entry Name
CHD3_HUMAN
NCBI Protein Information
chromodomain-helicase-DNA-binding protein 3; ATP-dependent helicase CHD3; CHD-3; hZFH; mi-2 autoantigen 240 kDa protein; zinc finger helicase; zinc-finger helicase (Snf2-like)

NCBI Summary for CHD3

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for CHD3

CHD-3 iso3: Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts (via its C-terminal) with HABP4. Interacts with PCNT; the interaction regulates centrosome integrity. Widely expressed. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Helicase; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; centrosome; intermediate filament cytoskeleton; cytoplasm; nucleolus; NuRD complex; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; zinc ion binding; helicase activity; ATP binding

Biological Process: chromatin assembly or disassembly; regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; centrosome organization and biogenesis; chromatin modification; spindle organization and biogenesis; DNA duplex unwinding
Show more

Research Articles on CHD3

1. CHD3.1 and ACF1-SNF2H display counteractive activities but similar histone affinities.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHD3 antibody

 Reference Product  PubMed Publications
 CHD4 antibody  >13 publications with CHD3 and CHD4
 SUMO1 antibody  >5 publications with CHD3 and SUMO1
 HDAC1 antibody  >5 publications with CHD3 and HDAC1
 MTA1 antibody  >4 publications with CHD3 and MTA1
 MBD2 antibody  >4 publications with CHD3 and MBD2
 HDAC2 antibody  >4 publications with CHD3 and HDAC2
 MTA2 antibody  >2 publications with CHD3 and MTA2
 RBBP4 antibody  >1 publications with CHD3 and RBBP4

Pathways associated with anti-CHD3 antibody

 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HDACs Deacetylate Histones Pathway antibodies  HDACs Deacetylate Histones Pathway Diagram
 RNA Polymerase I Promoter Clearance Pathway antibodies  RNA Polymerase I Promoter Clearance Pathway Diagram
 RNA Polymerase I Transcription Pathway antibodies  RNA Polymerase I Transcription Pathway Diagram
 RNA Polymerase I Transcription Initiation Pathway antibodies  RNA Polymerase I Transcription Initiation Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 Signaling Events Mediated By HDAC Class I Pathway antibodies  Signaling Events Mediated By HDAC Class I Pathway Diagram

Diseases associated with anti-CHD3 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >3 publications with CHD3 and Nervous System Diseases
 Heart Diseases Antibodies  >2 publications with CHD3 and Heart Diseases
 Death Antibodies  >1 publications with CHD3 and Death
 Neoplasm Metastasis Antibodies  >1 publications with CHD3 and Neoplasm Metastasis
 Movement Disorders Antibodies  >1 publications with CHD3 and Movement Disorders
 Fibrosis Antibodies  >1 publications with CHD3 and Fibrosis
 Inflammation Antibodies  >1 publications with CHD3 and Inflammation

Organs/Tissues associated with anti-CHD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >4 publications with CHD3 and Kidney
 Brain Antibodies  >4 publications with CHD3 and Brain
 Nerve Antibodies  >3 publications with CHD3 and Nerve
 Embryonic Tissue Antibodies  >2 publications with CHD3 and Embryonic Tissue
 Heart Antibodies  >2 publications with CHD3 and Heart
 Bone Antibodies  >2 publications with CHD3 and Bone
 Muscle Antibodies  >2 publications with CHD3 and Muscle
 Testis Antibodies  >1 publications with CHD3 and Testis
 Pancreas Antibodies  >1 publications with CHD3 and Pancreas
 Mouth Antibodies  >1 publications with CHD3 and Mouth
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000