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RFX1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3903349

Regulatory factor X, 1 (RFX1) is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory… More details

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Product Overview

Online Datasheet in PDF
Product Name
RFX1, Polyclonal Antibody
Full Product Name
Rabbit anti-RFX1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
MHC class II regulatory factor RFX; transcription factor RFX1; trans-acting regulatory factor 1; RFX; regulatory factor X, 1 (influences HLA class II expression); Regulatory factor X 1; enhancer factor C; EF-C; EFC; MHC class II regulatory factor RFX1; MHC class II regulatory factor RFX1; regulatory factor X1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
104,758 Da
OMIM
600006
3D Structure
ModBase 3D Structure for P22670
Immunogen
Between 200 and 250
Conjugation
Unconjugated

Product Categories/Family for anti-RFX1 antibody

Applications Tested/Suitable for anti-RFX1 antibody
Immunoprecipitation (IP)
Application Notes for anti-RFX1 antibody
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-RFX1 antibody

Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RFX1 antibody MBS3903349 used for IP at 6 ug/mg lysate. RFX1 was also immunoprecipitated by rabbit anti-RFX1 antibodies BL11050 and MBS3903350, which recognize other epitopes. For blotting immunoprecipitated RFX1, BL11050 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-RFX1 Polyclonal Antibody – Cat# MBS3903349

Other Notes

Small volumes of anti-RFX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RFX1 purchase

MBS3903349 is an antibody targeting RFX1. Also known as Rabbit anti-RFX1 Antibody, Affinity Purified. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RFX1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
MHC class II regulatory factor RFX1
NCBI Official Synonym Full Names
regulatory factor X1
NCBI Official Symbol
RFX1
NCBI Official Synonym Symbols
EFC; RFX
UniProt Protein Name
MHC class II regulatory factor RFX1
UniProt Synonym Protein Names
Enhancer factor C; EF-C; Regulatory factor X 1; RFX; Transcription factor RFX1
UniProt Gene Name
RFX1
UniProt Synonym Gene Names
EF-C; RFX
UniProt Entry Name
RFX1_HUMAN
NCBI Protein Information
MHC class II regulatory factor RFX1

NCBI Summary for RFX1

This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. [provided by RefSeq, Jul 2008]
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UniProt Comments for RFX1

RFX1: Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter. Belongs to the RFX family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function: protein binding; RNA polymerase II transcription factor activity, enhancer binding

Biological Process: immune response; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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