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NAT10 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3904573

N-acetyltransferase 10 (NAT10) has protein acetyltransferase activity in vitro. NAT10 can acetylate both histones and microtubules [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H0A0]. More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NAT10, Polyclonal Antibody
Full Product Name
Rabbit anti-NAT10 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
Kre33; RNA cytidine acetyltransferase; NET43; N-acetyltransferase-like protein; N-acetyltransferase 10 (GCN5-related); ALP; 18S rRNA cytosine acetyltransferase; N-acetyltransferase 10; RNA cytidine acetyltransferase; N-acetyltransferase 10
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
107,297 Da
OMIM
609221
3D Structure
ModBase 3D Structure for Q9H0A0
Immunogen
Between 675 and 725
Conjugation
Unconjugated

Product Categories/Family for anti-NAT10 antibody

Applications Tested/Suitable for anti-NAT10 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-NAT10 antibody
WB: 1:10,000-1:25,000
IP: 6ug/mg lysate
IHC: 1:500-1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-NAT10 antibody

Detection of mouse NAT10 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-NAT10 (Cat. No. MBS3904573 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-NAT10 Polyclonal Antibody – Cat# MBS3904573

Immunohistochemistry (IHC) of anti-NAT10 antibody

Detection of human NAT10 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NAT10 (Cat. No. MBS3904573 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-NAT10 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3904573

Immunoprecipitation (IP) of anti-NAT10 antibody

Detection of human NAT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody MBS3904573 (lot MBS3904573-1) used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by rabbit anti-NAT10 antibody BL15900. For blotting immunoprecipitated NAT10, MBS3904573 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-NAT10 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3904573

Western Blot (WB) of anti-NAT10 antibody

Detection of human NAT10 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody MBS3904573 (lot MBS3904573-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Rabbit Anti-NAT10 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3904573

Other Notes

Small volumes of anti-NAT10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NAT10 purchase

MBS3904573 is an antibody targeting NAT10. Also known as Rabbit anti-NAT10 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NAT10. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
N-acetyltransferase 10 isoform a
NCBI Official Synonym Full Names
N-acetyltransferase 10 (GCN5-related)
NCBI Official Symbol
NAT10
NCBI Official Synonym Symbols
ALP; NET43
UniProt Protein Name
N-acetyltransferase 10
UniProt Gene Name
NAT10
UniProt Synonym Gene Names
ALP; KIAA1709
UniProt Entry Name
NAT10_HUMAN
NCBI Protein Information
N-acetyltransferase 10; N-acetyltransferase-like protein

UniProt Comments for NAT10

ALP: Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division. Belongs to the UPF0202 family.

Protein type: EC 2.3.1.-; Acetyltransferase; Nucleolus

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: membrane; nucleolus; nucleus

Molecular Function: N-acetyltransferase activity; ATP binding

Biological Process: metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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