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WIPI2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3905463

WD repeat domain phosphoinositide-interacting protein 2 (WIPI2) is an early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
WIPI2, Polyclonal Antibody
Full Product Name
Rabbit anti-WIPI2 Antibody, Affinity Purified
Product Gene Name
anti-WIPI2 antibody
Product Synonym Names
Atg21; WIPI49-like protein 2; WIPI-2; WD40 repeat protein interacting with phosphoinositides 2; CGI-50; ATG18B; WD repeat domain phosphoinositide-interacting protein 2; WD repeat domain phosphoinositide-interacting protein 2; WD repeat domain, phosphoinositide interacting 2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
48,249 Da
OMIM
609225
3D Structure
ModBase 3D Structure for Q9Y4P8
Predicted Reactivity
Rat, Chicken
Immunogen
Between 404 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-WIPI2 antibody

Primary Antibodies
Applications Tested/Suitable for anti-WIPI2 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-WIPI2 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate

Immunoprecipitation (IP) of anti-WIPI2 antibody

Detection of human WIPI2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WIPI2 antibody MBS3905463 (lot MBS3905463-1) used for IP at 6 ug per reaction. WIPI2 was also immunoprecipitated by rabbit anti-WIPI2 antibody BL20890. For blotting immunoprecipitated WIPI2, MBS3905463 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-WIPI2 Polyclonal Antibody – Cat# MBS3905463

Western Blot (WB) of anti-WIPI2 antibody

Detection of human and mouse WIPI2 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WIPI2 antibody MBS3905463 (lot MBS3905463-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-WIPI2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3905463

Other Notes

Small volumes of anti-WIPI2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WIPI2 purchase

MBS3905463 is an antibody targeting WIPI2. Also known as Rabbit anti-WIPI2 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for WIPI2. It may not necessarily be applicable to this product.

NCBI GI #
54111428
NCBI GeneID
26100
NCBI Accession #
NP_057087.2
UniProt Primary Accession #
Q9Y4P8
UniProt Secondary Accession #
Q5MNZ8; Q6FI96; Q75L50; Q96IE4; Q9Y364; B3KNC2
UniProt Related Accession #
Q9Y4P8
GenBank Nucleotide #
NM_016003.3
NCBI Official Full Name
WD repeat domain phosphoinositide-interacting protein 2 isoform b
NCBI Official Synonym Full Names
WD repeat domain, phosphoinositide interacting 2
NCBI Official Symbol
WIPI2
NCBI Official Synonym Symbols
Atg21; ATG18B; CGI-50; WIPI-2
UniProt Protein Name
WD repeat domain phosphoinositide-interacting protein 2
UniProt Synonym Protein Names
WIPI49-like protein 2
Protein Family
WD repeat domain phosphoinositide-interacting protein
UniProt Gene Name
WIPI2
UniProt Synonym Gene Names
WIPI-2
UniProt Entry Name
WIPI2_HUMAN
NCBI Protein Information
WD repeat domain phosphoinositide-interacting protein 2

NCBI Summary for WIPI2

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008]
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UniProt Comments for WIPI2

WIPI2: Probable early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to PtdIns3P. May bind PtdIns3P. Interacts with TECPR1. Ubiquitously expressed. Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors. Belongs to the WD repeat SVP1 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 7p22.1

Cellular Component: nucleoplasm; protein complex; extrinsic to membrane; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; cytosol

Molecular Function: protein binding; phosphatidylinositol 3-phosphate binding

Biological Process: autophagic vacuole fusion; protein amino acid lipidation; mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation
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Research Articles on WIPI2

1. The specific autophagosomal localization of both WIPI1 and WIPI2 (refered to as WIPI puncta) has been employed to assess autophagy using fluorescence microscopy methods, such as confocal and live-cell video microscopy

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-WIPI2 antibody

 Reference Product  PubMed Publications
 ULK1 antibody  >4 publications with WIPI2 and ULK1
 ATG5 antibody  >4 publications with WIPI2 and ATG5
 ATG12 antibody  >3 publications with WIPI2 and ATG12
 ULK2 antibody  >1 publications with WIPI2 and ULK2
 ATG9A antibody  >1 publications with WIPI2 and ATG9A
 ATG2A antibody  >1 publications with WIPI2 and ATG2A

Pathways associated with anti-WIPI2 antibody

 Products by Pathway  Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Macroautophagy Pathway antibodies  Macroautophagy Pathway Diagram

Organs/Tissues associated with anti-WIPI2 antibody

 Organ/Tissue Name  Pubmed Publications
 Mouth Antibodies  >1 publications with WIPI2 and Mouth
 Embryonic Tissue Antibodies  >1 publications with WIPI2 and Embryonic Tissue
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