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MRLC2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS415377

MBS415377 is an antibody targeting MRLC2. Host: Mouse. More details

$540 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MRLC2, Monoclonal Antibody
Full Product Name
Anti-MRLC2 Antibody
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Form/Format
Liquid
Purity/Purification
Affinity purified
Concentration
100ug/100ul (lot specific)
Specificity
Anti-MRLC2 recognizes recombinant protein MRLC2.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Molecular Weight
19,779 Da
Raised In
Purified mouse monoclonal antibody generated using E.coli expressed recombinant protein MRLC2 as immunogen.
Storage Buffer
citrate-tris-HCl buffer pH7.0 with 0.02% Proclin 300.
Immunogen
Recombinant protein corresponding to full length MRLC2
Applications Tested/Suitable for anti-MRLC2 antibody
ELISA (EIA), Western Blot (WB-Re)
Application Notes for anti-MRLC2 antibody
WB: 1:1000 analysis of recombinant protein MRLC2 with Anti-MRLC2

Testing Data of anti-MRLC2 antibody

Mouse Anti-MRLC2 Monoclonal Antibody – Cat# MBS415377

Product References and Citations for anti-MRLC2 antibody

None

Other Notes

Small volumes of anti-MRLC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MRLC2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens myosin, light chain 12B, regulatory (MYL12B), transcript variant 2, mRNA
NCBI Official Synonym Full Names
myosin, light chain 12B, regulatory
NCBI Official Symbol
MYL12B
NCBI Official Synonym Symbols
MLC-B; MRLC2
UniProt Protein Name
Myosin regulatory light chain 12B
UniProt Synonym Protein Names
MLC-2A; MLC-2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa; MLC20; Myosin regulatory light chain MRLC2; SHUJUN-1
Protein Family
UniProt Gene Name
MYL12B
UniProt Entry Name
ML12B_HUMAN
NCBI Protein Information
myosin regulatory light chain 12B; MLC-2; MLC-2A; MLC20; SHUJUN-1; myosin regulatory light chain 2; myosin regulatory light chain 2-B, smooth muscle isoform; myosin regulatory light chain 20 kDa; myosin regulatory light chain MRLC2

NCBI Summary for MRLC2

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008]
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UniProt Comments for MRLC2

MRLC2: non-sarcomeric myosin regulatory light chain 2. Binds calcium and plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity. Phosphorylation of MLC-2 in the presence of calcium and calmodulin increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. Myosin is an hexamer of 2 heavy chains and 4 light chains.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 18p11.31

Cellular Component: myosin II complex; apical part of cell; stress fiber; cytosol; Z disc

Molecular Function: protein binding; calcium ion binding

Biological Process: regulation of cell shape; axon guidance; muscle contraction; ephrin receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MRLC2 antibody

Organs/Tissues associated with anti-MRLC2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >2 publications with MRLC2 and Muscle
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