APEX2 Antibody – Rabbit Polyclonal

APEX2: Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. Belongs to the DNA repair enzymes AP/ExoA family.

Protein type: Mitochondrial; EC 4.2.99.18; Lyase

Chromosomal Location of Human Ortholog: Xp11.21

Cellular Component: intracellular membrane-bound organelle; mitochondrial inner membrane; nucleolus; nucleus

Molecular Function: DNA-(apurinic or apyrimidinic site) lyase activity; DNA binding; zinc ion binding; double-stranded DNA specific 3'-5' exodeoxyribonuclease activity

Biological Process: base-excision repair; cell cycle; DNA catabolic process, exonucleolytic; DNA catabolic process, endonucleolytic; DNA recombination