Goat Anti-AGAP3 Polyclonal Antibody – Cat# MBS420354

(5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

CENTG3/MRIP1 (AGAP3) Antibody – Goat Polyclonal

Name: AGAP3 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420354
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420354

MBS420354 is an antibody targeting AGAP3. Also known as Goat anti-CENTG3/MRIP1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CENTG3/MRIP1 (AGAP3), Polyclonal Antibody

Full Product Name

Goat anti-CENTG3/MRIP1 Antibody

Product Gene Name

anti-AGAP3 antibody

Product Synonym Gene Name

CENTG3; MRIP1

Product Synonym Names

AGAP3; CENTG3; MRIP1; centaurin, gamma 3; MRIP-1; MRIP-1 protein; ArfGAP with GTPase domain, ankyrin repeat and PH domain 3; CRAG; FLJ16146; FLJ34452; CRMP (collapsin response mediator protein) associated; centaurin, gamma 3; AGAP3 antibody; CENTG3 antibody; MRIP1 antibody; centaurin; gamma 3 antibody; MRIP-1 antibody; MRIP-1 protein antibody; ArfGAP with GTPase domain; ankyrin repeat and PH domain 3 antibody; CRAG antibody; FLJ16146 antibody; FLJ34452 antibody; CRMP (collapsin response mediator protein) associated antibody; centaurin; gamma 3 antibody; CENTG3 / MRIP1

Immunogen Sequence

NPSAELHRSP SLL

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

CENTG3 / MRIP1 peptide (MBS426453) is used for blocking the activity of CENTG3/MRIP1 antibody (MBS420354)

Molecular Weight

42,517 Da

Immunogen

Peptide with sequence C-NPSAELHRSPSLL, from the C Terminus of the protein sequence according to NP_114152.3.

Epitope

C Terminus

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-AGAP3 antibody

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Application Notes for anti-AGAP3 antibody

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration, 5-10ug/ml.
Western Blot: Preliminary experiments gave an approx 45kDa band in Human Brain and Human Liver lysates at 1ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx.101kDda according to NP_114152.

Other Notes

Small volumes of anti-AGAP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AGAP3. It may not necessarily be applicable to this product.

NCBI GI #

110227613

NCBI GeneID

116988

NCBI Accession #

NP_114152.3

UniProt Secondary Accession #

Q59EN0; Q96RK3; B3KNZ8; E9PAL8

UniProt Related Accession #

Q96P47

GenBank Nucleotide #

NM_031946.6

NCBI Official Full Name

arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 isoform a

NCBI Official Synonym Full Names

ArfGAP with GTPase domain, ankyrin repeat and PH domain 3

NCBI Official Symbol

AGAP3

NCBI Official Synonym Symbols

CRAG; AGAP-3; CENTG3; MRIP-1; cnt-g3

UniProt Protein Name

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3

UniProt Synonym Protein Names

CRAM-associated GTPase; CRAG; Centaurin-gamma-3; Cnt-g3; MR1-interacting protein; MRIP-1

Protein Family

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein

UniProt Gene Name

AGAP3

UniProt Synonym Gene Names

CENTG3; AGAP-3; CRAG; Cnt-g3; MRIP-1

UniProt Entry Name

AGAP3_HUMAN

NCBI Protein Information

arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3

UniProt Comments for AGAP3

CENTG3: a member of the centaurin family of ADP-ribosylation factor directed (ARF) GTPase-activating proteins (GAPs). Protective against the pathogenesis of polyglutamine diseases. Mediates the nuclear translocation of the CENTG3-polyQ complexes and the formation of promyelocytic leukemia (PML) protein-associated nuclear bodies in reactive oxygen species (ROS) signaling. Promotes the degradation of polyQ proteins at PML nuclear bodies through the ubiquitin-proteasome pathway. Knockdown by small interfering RNA in neuronal cells consistently blocked the nuclear translocation of polyQ and enhanced polyQ-mediated cell death. Three alternatively-spliced isoforms have been described.

Protein type: GAPs; GAPs, ARF

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: cytoplasm; membrane

Molecular Function: GTP binding; GTPase activator activity; metal ion binding

Biological Process: positive regulation of GTPase activity; protein transport; small GTPase mediated signal transduction

Research Articles on AGAP3

1. AGAP3 is an essential signaling component of the NMDA receptor complex that links NMDA receptor activation to AMPA receptor trafficking.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-AGAP3 antibody

Products by Pathway	Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram

Diseases associated with anti-AGAP3 antibody

Disease Name	Pubmed Publications
Neurologic Manifestations Antibodies	>1 publications with AGAP3 and Neurologic Manifestations

Organs/Tissues associated with anti-AGAP3 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with AGAP3 and Brain