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RNF35/TRIM40 (TRIM40) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420482

MBS420482 is an antibody targeting TRIM40. Also known as Goat anti-RNF35/TRIM40 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RNF35/TRIM40, Polyclonal Antibody
Full Product Name
Goat anti-RNF35/TRIM40 Antibody
Product Gene Name
Product Synonym Gene Name
RNF35
Product Synonym Names
TRIM40; tripartite motif-containing 40; RNF35; ring finger RNF35; DAAP-200B17.3; ring finger protein 40; TRIM40 antibody; tripartite motif-containing 40 antibody; RNF35 antibody; ring finger RNF35 antibody; DAAP-200B17.3 antibody; ring finger protein 40 antibody; RNF35 / TRIM40
Immunogen Sequence
GVSELLLQPP QKL
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
26,073 Da
Immunogen
Peptide with sequence C-GVSELLLQPPQKL, from the C Terminus of the protein sequence according to NP_619645.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-TRIM40 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-TRIM40 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 26kDa band observed in Human Placenta lysates (calculated MW of 26.1kDa according to NP_619645.1). Recommended concentration: 0.5-2ug/ml.

Western Blot (WB) of anti-TRIM40 antibody

(0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-TRIM40 Polyclonal Antibody – Cat# MBS420482

Other Notes

Small volumes of anti-TRIM40 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIM40. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tripartite motif-containing protein 40 isoform b
NCBI Official Synonym Full Names
tripartite motif containing 40
NCBI Official Symbol
TRIM40
NCBI Official Synonym Symbols
RNF35
UniProt Protein Name
Tripartite motif-containing protein 40
UniProt Synonym Protein Names
Probable E3 NEDD8-protein ligase; RING finger protein 35
UniProt Gene Name
TRIM40
UniProt Synonym Gene Names
RNF35
UniProt Entry Name
TRI40_HUMAN
NCBI Protein Information
tripartite motif-containing protein 40

NCBI Summary for TRIM40

This gene encodes a member of the tripartite motif (TRIM) protein family. The encoded protein may play a role as a negative regulator against inflammation and carcinogenesis in the gastrointestinal tract. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Feb 2014]
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UniProt Comments for TRIM40

TRIM40: May function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway. Promotes neddylation of IKBKG/NEMO, stabilizing NFKBIA, and inhibiting of NF-kappaB nuclear translocation and activity. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: IkappaB kinase complex

Molecular Function: protein binding

Biological Process: inhibition of NF-kappaB transcription factor; negative regulation of protein catabolic process; protein neddylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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