• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Semaphorin 3e (SEMA3E) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420585

MBS420585 is an antibody targeting SEMA3E. Also known as Goat anti-Semaphorin 3E (internal) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Semaphorin 3E (SEMA3E), Polyclonal Antibody
Full Product Name
Goat anti-Semaphorin 3E (internal) Antibody
Product Gene Name
Product Synonym Names
Goat Anti-SEMA3E Antibody; SEMA3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; KIAA0331; M-SEMAH; M-SemaK; M-sema H; SEMAH; coll-5; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E; semaphorin 3E; SEMA3E antibody; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; (semaphorin) 3E antibody; KIAA0331 antibody; M-SEMAH antibody; M-SemaK antibody; M-sema H antibody; SEMAH antibody; coll-5 antibody; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; 3E antibody; semaphorin 3E antibody; Semaphorin 3E; Semaphorin 3E (internal)
Immunogen Sequence
NGGRYGTTKD YPDD
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
82,424 Da
OMIM
608166
Immunogen
Peptide with sequence C-NGGRYGTTKDYPDD, from the internal region of the protein sequence according to NP_036563.1; NP_001171600.1.
Epitope
Internal region
Note
This antibody is expected to recognise both reported isoforms (NP_036563.1; NP_001171600.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-SEMA3E antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-SEMA3E antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0. 03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2kDa according to NP_036563.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide.

Immunohistochemistry (IHC) of anti-SEMA3E antibody

(4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Goat Anti-SEMA3E Polyclonal Antibody – Cat# MBS420585

Other Notes

Small volumes of anti-SEMA3E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SEMA3E. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
semaphorin-3E isoform 1
NCBI Official Synonym Full Names
semaphorin 3E
NCBI Official Symbol
SEMA3E
NCBI Official Synonym Symbols
SEMAH; coll-5; M-SEMAH; M-SemaK
UniProt Protein Name
Semaphorin-3E
Protein Family
UniProt Gene Name
SEMA3E
UniProt Synonym Gene Names
KIAA0331; SEMAH
UniProt Entry Name
SEM3E_HUMAN
NCBI Protein Information
semaphorin-3E

NCBI Summary for SEMA3E

Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]
Show more

UniProt Comments for SEMA3E

SEMA3E: a secreted protein of the semaphorin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q21.11

Cellular Component: extracellular region; extracellular space

Molecular Function: chemorepellent activity; protein binding; semaphorin receptor binding

Biological Process: negative chemotaxis; negative regulation of angiogenesis; negative regulation of axon extension involved in axon guidance; negative regulation of cell-matrix adhesion; neural crest cell migration; patterning of blood vessels; positive regulation of cell migration; regulation of cell shape; sprouting angiogenesis; synapse organization and biogenesis

Disease: Charge Syndrome
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000