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ELF1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420587

MBS420587 is an antibody targeting ELF1. Also known as Goat anti-ELF1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ELF1, Polyclonal Antibody
Full Product Name
Goat anti-ELF1 Antibody
Product Gene Name
Product Synonym Names
ELF1; E74-like factor 1 (ets domain transcription factor); OTTHUMP00000018310; ets-related transcription factor Elf-1; ELF1 antibody; E74-like factor 1 (ets domain transcription factor) antibody; OTTHUMP00000018310 antibody; ets-related transcription factor Elf-1 antibody
Immunogen Sequence
AMKQNELLEP NSF
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
ELF1 peptide (MBS426820) is used for blocking the activity of ELF1 antibody (MBS420587)
Molecular Weight
64,929 Da
OMIM
189973
Immunogen
Peptide with sequence C-AMKQNELLEPNSF, from the C Terminus of the protein sequence according to NP_758961.1; NP_001138825.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-ELF1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ELF1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 70kDa band observed in Human Tonsil, Lymph Node and in Jurkat T-cell line lysates (calculated MW of 67.5kDa according to NP_758961.1). Recommended concentration: 0.3-1. 0ug/ml.

Western Blot (WB) of anti-ELF1 antibody

(0.3ug/ml) staining of Human Lymph Node (A), Human Tonsil (B) and Mouse Thymus (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ELF1 Polyclonal Antibody – Cat# MBS420587

Western Blot (WB) of anti-ELF1 antibody

(0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ELF1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS420587

Other Notes

Small volumes of anti-ELF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ELF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ETS-related transcription factor Elf-1 isoform a
NCBI Official Synonym Full Names
E74 like ETS transcription factor 1
NCBI Official Symbol
ELF1
UniProt Protein Name
ETS-related transcription factor Elf-1
UniProt Synonym Protein Names
E74-like factor 1
UniProt Gene Name
ELF1
UniProt Entry Name
ELF1_HUMAN
NCBI Protein Information
ETS-related transcription factor Elf-1

NCBI Summary for ELF1

This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]
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UniProt Comments for ELF1

Elf-1: Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. Belongs to the ETS family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 13q13

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; transcription factor activity

Biological Process: cell differentiation; negative regulation of T cell receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of cytokine production; transcription from RNA polymerase II promoter
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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