Goat Anti-VPS41 Polyclonal Antibody – Cat# MBS420935

(4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.

VPS41 Antibody – Goat Polyclonal

Name: VPS41 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS420935
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS420935

MBS420935 is an antibody targeting VPS41. Also known as Goat anti-VPS41 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

VPS41, Polyclonal Antibody

Full Product Name

Goat anti-VPS41 Antibody

Product Gene Name

anti-VPS41 antibody

Product Synonym Names

VPS41; vacuolar protein sorting 41 (yeast); HGNC: 12713; HVSP41; vacuolar assembly protein 41; vacuolar protein sorting 41 (yeast homolog); vacuolar protein sorting 41 homolog (S. cerevisiae); HVPS41; hVps41p; vacuolar protein sorting 41; VPS41 antibody; vacuolar protein sorting 41 (yeast) antibody; HGNC: 12713 antibody; HVSP41 antibody; vacuolar assembly protein 41 antibody; vacuolar protein sorting 41 (yeast homolog) antibody; vacuolar protein sorting 41 homolog (S. cerevisiae) antibody; HVPS41 antibody; hVps41p antibody; vacuolar protein sorting 41 antibody

Immunogen Sequence

DHLKKDSQNK TLLK

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

VPS41 peptide (MBS426666) is used for blocking the activity of VPS41 antibody (MBS420935)

Molecular Weight

95,924 Da

OMIM

605485

Immunogen

Peptide with sequence C-DHLKKDSQNKTLLK, from the internal region of the protein sequence according to NP_055211.2; NP_542198.2.

Epitope

Internal region

Note

This antibody is expected to recognise both reported isoforms (NP_055211.2 and NP_542198.2)

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-VPS41 antibody

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Application Notes for anti-VPS41 antibody

Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Kidney shows staining of collective tubules. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave bands at approx 48kDa and 28kDa in human duodenum, kidney and placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 98.5kDa according to NP_055211.1 and 100kDa according to NP_542198.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Have any further splice variants/modified forms been reported.
IHC: In paraffin embedded Human Kidney shows staining of collective tubules. Recommended concentration, 4-6ug/ml.

Other Notes

Small volumes of anti-VPS41 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VPS41. It may not necessarily be applicable to this product.

NCBI GI #

114199475

NCBI GeneID

27072

NCBI Accession #

NP_055211.2

NCBI Related Accession #

NP_542198.2

UniProt Secondary Accession #

Q86TP8; Q99851; Q99852; E9PF36

UniProt Related Accession #

P49754

GenBank Nucleotide #

NM_014396.3

NCBI Official Full Name

vacuolar protein sorting-associated protein 41 homolog isoform 1

NCBI Official Synonym Full Names

VPS41, HOPS complex subunit

NCBI Official Symbol

VPS41

NCBI Official Synonym Symbols

HVPS41; HVSP41; hVps41p

UniProt Protein Name

Vacuolar protein sorting-associated protein 41 homolog

UniProt Synonym Protein Names

S53

Protein Family

Vacuolar protein sorting-associated protein

UniProt Gene Name

VPS41

UniProt Entry Name

VPS41_HUMAN

NCBI Protein Information

vacuolar protein sorting-associated protein 41 homolog

NCBI Summary for VPS41

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human ortholog of yeast Vps41 protein which is also conserved in Drosophila, tomato, and Arabidopsis. Expression studies in yeast and human indicate that this protein may be involved in the formation and fusion of transport vesicles from the Golgi. Several transcript variants encoding different isoforms have been described for this gene, however, the full-length nature of not all is known. [provided by RefSeq, Jul 2008]

UniProt Comments for VPS41

VPS41: Required for vacuolar assembly and vacuolar traffic. Belongs to the VPS41 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 7p14-p13

Cellular Component: clathrin-coated vesicle; cytosol; early endosome; endosome membrane; Golgi-associated vesicle; late endosome membrane; lysosomal membrane; membrane; microtubule cytoskeleton

Molecular Function: GTPase binding; identical protein binding; microtubule binding; protein binding; protein self-association; zinc ion binding

Biological Process: autophagy; endosomal vesicle fusion; endosome to lysosome transport; Golgi vesicle transport; protein targeting to vacuole; regulated secretory pathway; vacuole fusion, non-autophagic

Research Articles on VPS41

1. VPS41 subunit of HOPS complex was defined to be the major partner for interacting with RILP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-VPS41 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>2 publications with VPS41 and Nervous System Diseases
Movement Disorders Antibodies	>2 publications with VPS41 and Movement Disorders
Learning Disorders Antibodies	>1 publications with VPS41 and Learning Disorders
Disease Models, Animal Antibodies	>1 publications with VPS41 and Disease Models, Animal
Weight Loss Antibodies	>1 publications with VPS41 and Weight Loss

Organs/Tissues associated with anti-VPS41 antibody

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>3 publications with VPS41 and Eye
Nerve Antibodies	>2 publications with VPS41 and Nerve
Bone Antibodies	>2 publications with VPS41 and Bone
Blood Antibodies	>2 publications with VPS41 and Blood
Lung Antibodies	>1 publications with VPS41 and Lung
Placenta Antibodies	>1 publications with VPS41 and Placenta
Skin Antibodies	>1 publications with VPS41 and Skin
Embryonic Tissue Antibodies	>1 publications with VPS41 and Embryonic Tissue
Stomach Antibodies	>1 publications with VPS41 and Stomach
Liver Antibodies	>1 publications with VPS41 and Liver