• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Stearoyl-Coa Desaturase (SCD) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421254

MBS421254 is an antibody targeting SCD. Also known as Goat anti-Stearoyl-CoA desaturase Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Stearoyl-CoA desaturase (SCD), Polyclonal Antibody
Full Product Name
Goat anti-Stearoyl-CoA desaturase Antibody
Product Gene Name
Product Synonym Names
SCD; SCD1; HGNC: 10571; FADS5; PRO0998; acyl-CoA desaturase; delta-9-desaturase; fatty acid desaturase; predicted protein of HQ0998; stearoyl-CoA desaturase; SCD antibody; SCD1 antibody; HGNC: 10571 antibody; FADS5 antibody; PRO0998 antibody; acyl-CoA desaturase antibody; delta-9-desaturase antibody; fatty acid desaturase antibody; predicted protein of HQ0998 antibody; stearoyl-CoA desaturase antibody; Stearoyl-CoA desaturase; Stearoyl-CoA desaturase
Immunogen Sequence
RIKRTGDGNY KSG
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
OMIM
604031
Immunogen
Peptide with sequence C-RIKRTGDGNYKSG, from the C Terminus of the protein sequence according to NP_005054.3.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-SCD antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-SCD antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplam staining in hepatocytes. Recommended concentration, 5-10ug/ml.
Western Blot: Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_005054.3. The 30kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Liver and Colon. Recommended concentration: 5ug/ml.
Flow Cytometry: Exclusively staining of HepG2 when mixed with Peripheral Blood Lymphocytes.

Immunohistochemistry (IHC) of anti-SCD antibody

(5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-SCD Polyclonal Antibody – Cat# MBS421254

Immunohistochemistry (IHC) of anti-SCD antibody

MBS421254 (5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-SCD Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS421254

Flow Cytometry (FC) of anti-SCD antibody

MBS421254 (2.5ul) staining of HepG2 cells mixed in with PBL. Detected by fluorescence with PE for SCD in green and with OC515 for CD45 in red.
Goat Anti-SCD Polyclonal Antibody, Flow Cytometry (FC) – Cat# MBS421254

Other Notes

Small volumes of anti-SCD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SCD. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
acyl-CoA desaturase
NCBI Official Synonym Full Names
stearoyl-CoA desaturase (delta-9-desaturase)
NCBI Official Symbol
SCD
NCBI Official Synonym Symbols
SCD1; FADS5; SCDOS; hSCD1; MSTP008
UniProt Protein Name
Acyl-CoA desaturase
UniProt Synonym Protein Names
Delta(9)-desaturase; Delta-9 desaturase; Fatty acid desaturase; Stearoyl-CoA desaturase
Protein Family
UniProt Gene Name
SCD
UniProt Synonym Gene Names
hSCD1
UniProt Entry Name
ACOD_HUMAN
NCBI Protein Information
acyl-CoA desaturase

NCBI Summary for SCD

This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Sep 2015]
Show more

UniProt Comments for SCD

SCD: Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Belongs to the fatty acid desaturase family.

Protein type: Endoplasmic reticulum; Membrane protein, integral; EC 1.14.19.1; Membrane protein, multi-pass; Lipid Metabolism - unsaturated fatty acid biosynthesis; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q24.31

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; membrane

Molecular Function: iron ion binding; oxidoreductase activity; stearoyl-CoA 9-desaturase activity

Biological Process: unsaturated fatty acid biosynthetic process
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000