Goat Anti-RIC8A Polyclonal Antibody – Cat# MBS421357

Staining (1ug/ml) of human brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

RIC8A Antibody – Goat Polyclonal

Name: RIC8A Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421357
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421357

MBS421357 is an antibody targeting RIC8A. Also known as Goat anti-RIC8A/Synembryn Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RIC8A/Synembryn, Polyclonal Antibody

Full Product Name

Goat anti-RIC8A/Synembryn Antibody

Product Gene Name

anti-RIC8A antibody

Product Synonym Names

RIC8A; synembryn; resistance to inhibitors of cholinesterase 8 homolog A (C. elegans); MGC104517; MGC131931; RIC8; RIC8 protein; resistance to inhibitors of cholinesterase 8 homolog A; MGC148073; MGC148074; RIC8A antibody; synembryn antibody; resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) antibody; MGC104517 antibody; MGC131931 antibody; RIC8 antibody; RIC8 protein antibody; resistance to inhibitors of cholinesterase 8 homolog A antibody; Synembryn; RIC8A / Synembryn

Immunogen Sequence

CLSSDPDSDP D

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

RIC8A / Synembryn peptide (MBS425503) is used for blocking the activity of RIC8A/Synembryn antibody (MBS421357)

Molecular Weight

59,612 Da

OMIM

609146

Immunogen

Peptide with sequence CLSSDPDSDPD, from the C Terminus of the protein sequence according to NP_068751.4.

Epitope

C Terminus

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-RIC8A antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-RIC8A antibody

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 70kDa band observed in human brain lysates (calculated MW of 60.4kDa according to NP_068751.4). Recommended concentration: 1-3ug/ml.

Other Notes

Small volumes of anti-RIC8A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RIC8A. It may not necessarily be applicable to this product.

NCBI GI #

27883866

NCBI GeneID

60626

NCBI Accession #

NP_068751.4

UniProt Secondary Accession #

Q0P508; Q2T9J1; Q7Z352; Q96EZ1; Q96SZ2; Q9H064; Q9H5H3; Q9H9E7

UniProt Related Accession #

Q9NPQ8

GenBank Nucleotide #

NM_021932.5

NCBI Official Full Name

synembryn-A isoform 1

NCBI Official Synonym Full Names

RIC8 guanine nucleotide exchange factor A

NCBI Official Symbol

RIC8A

NCBI Official Synonym Symbols

RIC8

UniProt Protein Name

Synembryn-A

UniProt Synonym Protein Names

Protein Ric-8A

Protein Family

Synembryn

UniProt Gene Name

RIC8A

UniProt Entry Name

RIC8A_HUMAN

NCBI Protein Information

synembryn-A

UniProt Comments for RIC8A

RIC8A: Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation. Belongs to the synembryn family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, misc.

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; plasma membrane

Molecular Function: G-protein alpha-subunit binding; GTPase activator activity; guanyl-nucleotide exchange factor activity; protein binding

Biological Process: cell migration involved in gastrulation; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; in utero embryonic development; positive regulation of GTPase activity; vasculature development; visual learning

Research Articles on RIC8A

1. Human NCS-1 and Ric8a reproduce the binding and maintain the structural requirements at these key positions. Drosophila Ric8a and Galphas regulate synapse number and neurotransmitter release, and both are functionally linked to Frq2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RIC8A antibody

Reference Product	PubMed Publications
GNAI1 antibody	>1 publications with RIC8A and GNAI1

Diseases associated with anti-RIC8A antibody

Disease Name	Pubmed Publications
Neurologic Manifestations Antibodies	>1 publications with RIC8A and Neurologic Manifestations

Organs/Tissues associated with anti-RIC8A antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>5 publications with RIC8A and Brain
Kidney Antibodies	>2 publications with RIC8A and Kidney
Heart Antibodies	>1 publications with RIC8A and Heart
Ovary Antibodies	>1 publications with RIC8A and Ovary
Muscle Antibodies	>1 publications with RIC8A and Muscle
Embryonic Tissue Antibodies	>1 publications with RIC8A and Embryonic Tissue