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WIPF1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421377

MBS421377 is an antibody targeting WIPF1. Also known as Goat anti-WIPF1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

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Product Overview

Product Name
WIPF1, Polyclonal Antibody
Full Product Name
Goat anti-WIPF1 Antibody
Product Gene Name
anti-WIPF1 antibody
Product Synonym Names
WIPF1; WAS/WASL interacting protein family, member 1; MGC111041; PRPL-2; WASPIP; WIP; Wiskott-Aldrich syndrome protein interacting protein; WIPF1 antibody; WAS/WASL interacting protein family; member 1 antibody; MGC111041 antibody; PRPL-2 antibody; WASPIP antibody; WIP antibody; Wiskott-Aldrich syndrome protein interacting protein antibody
Immunogen Sequence
KLARNESRSG SNRRE
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
36,426 Da
OMIM
602357
Immunogen
Peptide with sequence C-KLARNESRSGSNRRE, from the C Terminus of the protein sequence according to NP_003378.3; NP_001070737.1.
Epitope
C Terminus
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-WIPF1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-WIPF1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx.55kDa band observed in Human Lymph Node, Spleen and Tonsil lysates (calculated MW of 51.3kDa according to NP_003378.3 and NP_001070737.1). In transfected HEK293 transiently expressing WIPF1 a band of approx.55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 03-0.1ug/ml.

Western Blot (WB) of anti-WIPF1 antibody

(0. 03ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-WIPF1 Polyclonal Antibody – Cat# MBS421377

Western Blot (WB) of anti-WIPF1 antibody

HEK293 overexpressing WIPF1 (RC217601) and probed (mock transfection in first lane), tested by Origene.
Goat Anti-WIPF1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS421377

Other Notes

Small volumes of anti-WIPF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WIPF1. It may not necessarily be applicable to this product.

NCBI GI #
38373695
NCBI GeneID
7456
NCBI Accession #
NP_003378.3
NCBI Related Accession #
NP_001070737.1
UniProt Secondary Accession #
Q15220; Q53TA9; Q6MZU9; Q9BU37; Q9UNP1; B8ZZM1; D3DPE4
UniProt Related Accession #
O43516
GenBank Nucleotide #
NM_003387.4
NCBI Official Full Name
WAS/WASL-interacting protein family member 1
NCBI Official Synonym Full Names
WAS/WASL interacting protein family member 1
NCBI Official Symbol
WIPF1
NCBI Official Synonym Symbols
WIP; WAS2; PRPL-2; WASPIP
UniProt Protein Name
WAS/WASL-interacting protein family member 1
UniProt Synonym Protein Names
Protein PRPL-2; Wiskott-Aldrich syndrome protein-interacting protein; WASP-interacting protein
Protein Family
WAS/WASL-interacting protein family
UniProt Gene Name
WIPF1
UniProt Synonym Gene Names
WASPIP; WIP; WASP-interacting protein
UniProt Entry Name
WIPF1_HUMAN
NCBI Protein Information
WAS/WASL-interacting protein family member 1

NCBI Summary for WIPF1

This gene encodes a protein that plays an important role in the organization of the actin cytoskeleton. The encoded protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these two proteins may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for WIPF1

WIPF1: a cytoskeleton-associated protein that interacts with the Wiskott-Aldrich syndrome protein (WASP) in resting cells. Plays an important role in the organization of the actin cytoskeleton. Phosphorylation by PKC-theta following T cell receptor signaling disengages it from WASP, releasing WASP from WIP inhibition. Binds to a region of WASP that is frequently mutated in Wiskott-Aldrich syndrome.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: actin cytoskeleton; actin filament; cytoplasmic membrane-bound vesicle; cytosol; ruffle

Molecular Function: actin binding; actin filament binding; profilin binding; protein binding

Biological Process: actin cortical patch assembly; actin cortical patch localization; actin filament-based movement; actin polymerization and/or depolymerization; endocytosis; positive regulation of actin nucleation; protein complex assembly; regulation of cell shape; response to other organism

Disease: Wiskott-aldrich Syndrome 2
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Research Articles on WIPF1

1. conclude that tyrosine phosphorylation of WIP is a crucial regulator of WASP stability and function as an actin-nucleation-promoting factor

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-WIPF1 antibody

 Reference Product  PubMed Publications
 WASL antibody  >14 publications with WIPF1 and WASL
 CDC42 antibody  >13 publications with WIPF1 and CDC42
 SYK antibody  >3 publications with WIPF1 and SYK
 ACTR3 antibody  >3 publications with WIPF1 and ACTR3
 ACTR2 antibody  >3 publications with WIPF1 and ACTR2
 CTTN antibody  >2 publications with WIPF1 and CTTN

Pathways associated with anti-WIPF1 antibody

 Products by Pathway  Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies  Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
 Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway antibodies  Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 RHO GTPase Effectors Pathway antibodies  RHO GTPase Effectors Pathway Diagram
 RHO GTPases Activate WASPs And WAVEs Pathway antibodies  RHO GTPases Activate WASPs And WAVEs Pathway Diagram
 Regulation Of Actin Dynamics For Phagocytic Cup Formation Pathway antibodies  Regulation Of Actin Dynamics For Phagocytic Cup Formation Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram

Diseases associated with anti-WIPF1 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >4 publications with WIPF1 and Breast Neoplasms
 Nervous System Diseases Antibodies  >3 publications with WIPF1 and Nervous System Diseases
 Brain Diseases Antibodies  >2 publications with WIPF1 and Brain Diseases
 Leukemia Antibodies  >1 publications with WIPF1 and Leukemia
 Neoplasm Invasiveness Antibodies  >1 publications with WIPF1 and Neoplasm Invasiveness
 Cell Transformation, Neoplastic Antibodies  >1 publications with WIPF1 and Cell Transformation, Neoplastic
 Neoplasm Metastasis Antibodies  >1 publications with WIPF1 and Neoplasm Metastasis
 Inflammation Antibodies  >1 publications with WIPF1 and Inflammation
 Necrosis Antibodies  >1 publications with WIPF1 and Necrosis

Organs/Tissues associated with anti-WIPF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >22 publications with WIPF1 and Blood
 Nerve Antibodies  >12 publications with WIPF1 and Nerve
 Brain Antibodies  >5 publications with WIPF1 and Brain
 Bone Antibodies  >4 publications with WIPF1 and Bone
 Bone Marrow Antibodies  >3 publications with WIPF1 and Bone Marrow
 Vascular Antibodies  >2 publications with WIPF1 and Vascular
 Heart Antibodies  >1 publications with WIPF1 and Heart
 Mammary Gland Antibodies  >1 publications with WIPF1 and Mammary Gland
 Spleen Antibodies  >1 publications with WIPF1 and Spleen
 Lung Antibodies  >1 publications with WIPF1 and Lung
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