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GFP Antibody – Rabbit

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS462051

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters,… More details

$335 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GFP, Antibody
Full Product Name
Rabbit anti GFP Polyclonal antibody
Product Gene Name
Product Synonym Names
Rabbit anti-GFP; Green Fluorescent Protein
Isotype
IgG
Host
Rabbit
Species Reactivity
GFP-Tag
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
Purity/Purification
The Rabbit IgG is purified by Epitope Affinity Purification
Specificity
This antibody recognizes ~ 26 kDa of Green Fluorescent Protein. It is a tag antibody.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2°C to 8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
26.0
Antigen Preparation
A full length GFP recombinant protein.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Applications Tested/Suitable for anti-GFP antibody
Western Blot (WB), ELISA, Immunoprecipitation (IP)
Application Notes for anti-GFP antibody
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
IHC: 2-10 ug/ml
Flow cytometry: Not tested

Western Blot of anti-GFP antibody

Western Blot: The recombinant protein GFP (10 ug/lane) immuneblotted by Rabbit anti- GFP antibody (Cat#MBS462051) at 1:1000. Observed a major immunoreactive band at molecular weight ~26 kDa.
Rabbit Anti-GFP Antibody – Cat# MBS462051

Product References and Citations for anti-GFP antibody

Prendergast F, Mann K (1978). "Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskålea". Biochemistry 17 (17): 3448-53

Other Notes

Small volumes of anti-GFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GFP purchase

MBS462051 is an antibody targeting GFP. Also known as Rabbit anti GFP Polyclonal antibody. Reactive in GFP-Tag. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GFP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
GFP

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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