Goat Anti-SETDB2 Polyclonal Antibody – Cat# MBS421496

(1.5ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CLLD8/SETDB2 (SETDB2) Antibody – Goat Polyclonal

Name: SETDB2 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421496
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421496

MBS421496 is an antibody targeting SETDB2. Also known as Goat anti-CLLD8/SETDB2 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CLLD8/SETDB2, Polyclonal Antibody

Full Product Name

Goat anti-CLLD8/SETDB2 Antibody

Product Gene Name

anti-SETDB2 antibody

Product Synonym Gene Name

CLLD8

Product Synonym Names

Goat Anti-SETDB2 Antibody; SETDB2; CLLD8; SET domain, bifurcated 2; CLLL8 protein; C13orf4; CLLL8; DKFZp586I0123; DKFZp761J1217; KMT1F; chronic lymphocytic leukemia deletion region 8; lysine N-methyltransferase 1F; histone-lysine N-methyltransferase SETDB2; SETDB2 antibody; CLLD8 antibody; SET domain; bifurcated 2 antibody; CLLL8 protein antibody; C13orf4 antibody; CLLL8 antibody; DKFZp586I0123 antibody; DKFZp761J1217 antibody; KMT1F antibody; chronic lymphocytic leukemia deletion region 8 antibody; lysine N-methyltransferase 1F antibody; histone-lysine N-methyltransferase SETDB2 antibody; CLLD8 / SETDB2

Immunogen Sequence

GEKNGDAKTF WME

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

CLLD8 / SETDB2 peptide (MBS428287) is used for blocking the activity of CLLD8/SETDB2 antibody (MBS421496)

Molecular Weight

81,766 Da

OMIM

607865

Immunogen

Peptide with sequence GEKNGDAKTFWME-C, from the N Terminus of the protein sequence according to NP_114121.2; NP_001153780.1.

Epitope

N Terminus

Note

This antibody is expected to recognize both reported isoforms (NP_114121.2; NP_001153780.1).

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-SETDB2 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-SETDB2 antibody

Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 80kDa band observed in Human Heart lysates (calculated MW of 81.9kDa according to NP_114121.1). Recommended concentration: 1.5-4.5ug/ml. This product is not suitable in WB on Mouse Heart and Rat Heart lysates.

Other Notes

Small volumes of anti-SETDB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SETDB2. It may not necessarily be applicable to this product.

NCBI GI #

238624095

NCBI GeneID

83852

NCBI Accession #

NP_114121.2

NCBI Related Accession #

NP_001153780.1

UniProt Secondary Accession #

Q5TC65; Q5TC66; Q5W0A7; Q659A7; Q86UD6; Q96AI6

UniProt Related Accession #

Q96T68

GenBank Nucleotide #

NM_031915.2

NCBI Official Full Name

histone-lysine N-methyltransferase SETDB2 isoform a

NCBI Official Synonym Full Names

SET domain bifurcated 2

NCBI Official Symbol

SETDB2

NCBI Official Synonym Symbols

CLLD8; CLLL8; KMT1F; C13orf4

UniProt Protein Name

Histone-lysine N-methyltransferase SETDB2

UniProt Synonym Protein Names

Chronic lymphocytic leukemia deletion region gene 8 protein; Lysine N-methyltransferase 1F; SET domain bifurcated 2

Protein Family

Histone-lysine N-methyltransferase

UniProt Gene Name

SETDB2

UniProt Synonym Gene Names

C13orf4; CLLD8; KMT1F

UniProt Entry Name

SETB2_HUMAN

NCBI Protein Information

histone-lysine N-methyltransferase SETDB2

NCBI Summary for SETDB2

This gene encodes a member of a family of proteins that contain a methyl-CpG-binding domain (MBD) and a SET domain and function as histone methyltransferases. This protein is recruited to heterochromatin and plays a role in the regulation of chromosome segregation. This region is commonly deleted in chronic lymphocytic leukemia. Naturally-occuring readthrough transcription occurs from this gene to the downstream PHF11 (PHD finger protein 11) gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

UniProt Comments for SETDB2

SETDB2: Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis. Belongs to the histone-lysine methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: chromosome; nucleoplasm; nucleus

Molecular Function: DNA binding; histone lysine N-methyltransferase activity (H3-K9 specific); histone-lysine N-methyltransferase activity; protein binding; zinc ion binding

Biological Process: cell division; chromosome segregation; heart looping; histone H3-K9 methylation; mitosis; negative regulation of transcription, DNA-dependent

Research Articles on SETDB2

1. these findings identify Setdb2 as a novel regulator of the immune system in acute respiratory viral infection.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SETDB2 antibody

Products by Pathway	Pathway Diagram
Chromatin Modifying Enzymes Pathway antibodies	Chromatin Modifying Enzymes Pathway Diagram
Chromatin Organization Pathway antibodies	Chromatin Organization Pathway Diagram
Lysine Degradation Pathway antibodies	Lysine Degradation Pathway Diagram
Lysine Degradation Pathway antibodies	Lysine Degradation Pathway Diagram
PKMTs Methylate Histone Lysines Pathway antibodies	PKMTs Methylate Histone Lysines Pathway Diagram

Diseases associated with anti-SETDB2 antibody

Disease Name	Pubmed Publications
Lung Diseases Antibodies	>3 publications with SETDB2 and Lung Diseases
Stomach Neoplasms Antibodies	>1 publications with SETDB2 and Stomach Neoplasms
Leukemia Antibodies	>1 publications with SETDB2 and Leukemia
Chromosome Aberrations Antibodies	>1 publications with SETDB2 and Chromosome Aberrations
Breast Neoplasms Antibodies	>1 publications with SETDB2 and Breast Neoplasms
Inflammation Antibodies	>1 publications with SETDB2 and Inflammation

Organs/Tissues associated with anti-SETDB2 antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>3 publications with SETDB2 and Lung
Blood Antibodies	>2 publications with SETDB2 and Blood
Heart Antibodies	>2 publications with SETDB2 and Heart
Testis Antibodies	>1 publications with SETDB2 and Testis