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GPR3 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421547

MBS421547 is an antibody targeting GPR3. Also known as Goat anti-GPR3 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GPR3, Polyclonal Antibody
Full Product Name
Goat anti-GPR3 Antibody
Product Gene Name
Product Synonym Names
GPR3; G protein-coupled receptor 3; ACCA; OTTHUMP00000043209; adenylate cyclase constitutive activator; GPR3 antibody; G protein-coupled receptor 3 antibody; ACCA antibody; OTTHUMP00000043209 antibody; adenylate cyclase constitutive activator antibody
Immunogen Sequence
LQRHLLPASH
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
GPR3 peptide (MBS426020) is used for blocking the activity of GPR3 antibody (MBS421547)
Molecular Weight
35,010 Da
OMIM
600241
Immunogen
Peptide with sequence C-LQRHLLPASH, from the internal region of the protein sequence according to NP_005272.1.
Epitope
Internal region
Note
This antibody is not expected to cross-react with GPR6 and GPR12.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-GPR3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-GPR3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 35kDa band observed in Human Brain (Hippocampus and Frontal Cortex) lysates (calculated MW of 35. 0kDa according to NP_005272.1). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 30kDa.

Western Blot (WB) of anti-GPR3 antibody

(0.3ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-GPR3 Polyclonal Antibody – Cat# MBS421547

Other Notes

Small volumes of anti-GPR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GPR3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
G-protein coupled receptor 3
NCBI Official Synonym Full Names
G protein-coupled receptor 3
NCBI Official Symbol
GPR3
NCBI Official Synonym Symbols
ACCA
UniProt Protein Name
G-protein coupled receptor 3
UniProt Synonym Protein Names
ACCA orphan receptor
UniProt Gene Name
GPR3
UniProt Synonym Gene Names
ACCA
UniProt Entry Name
GPR3_HUMAN
NCBI Protein Information
G-protein coupled receptor 3

NCBI Summary for GPR3

This gene is a member of the G protein-coupled receptor family and is found in the cell membrane. G protein-coupled receptors, characterized by a seven transmembrane domain motif, are involved in translating outside signals into G protein mediated intracellular effects. The encoded protein activates adenylate cyclase and modulates amyloid-beta production in a mouse model, suggesting that it may play a role in Alzheimer's disease. [provided by RefSeq, Oct 2012]
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UniProt Comments for GPR3

GPR3: Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress, amyloid-beta peptide generation in neurons and neurite outgrowth. Maintains also meiotic arrest in oocytes. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, integral; Cell cycle regulation; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: integral to plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding

Biological Process: G-protein signaling, adenylate cyclase activating pathway; regulation of meiosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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