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ABCB5 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421569

MBS421569 is an antibody targeting ABCB5. Also known as Goat anti-ABCB5 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ABCB5, Polyclonal Antibody
Full Product Name
Goat anti-ABCB5 Antibody
Product Gene Name
anti-ABCB5 antibody
Product Synonym Names
Goat Anti-ABCB5 (aa364-378) Antibody; ATP-binding cassette, sub-family B (MDR/TAP), member 5; ABCB5alpha; ABCB5beta; EST422562; ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5; ATP-binding cassette; sub-family B (MDR/TAP); member 5 antibody; ABCB5alpha antibody; ABCB5beta antibody; EST422562 antibody; ATP-binding cassette; sub-family B; member 5N: P-glycoprotein ABCB5 antibody
Immunogen Sequence
QTQHRNTSKK AQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Antibody/Peptide Pairs
ABCB5 peptide (MBS428014) is used for blocking the activity of ABCB5 antibody (MBS421569)
OMIM
611785
Immunogen
Peptide with sequence C-QTQHRNTSKKAQ, from the internal region of the protein sequence according to NP_848654.3; NP_001157413.1.
Epitope
Internal region
Note
This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-ABCB5 antibody
Peptide ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-ABCB5 antibody
Peptide ELISA: antibody detection limit dilution 1:32000.

Western blot: Approx 80kDa band observed in lysates of cell line A431(calculated MW of 89.8kDa according to NP_848654.3).
Recommended concentration: 0.3-1ug/ml.
Primary incubation was 1 hour.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Western Blot (WB) of anti-ABCB5 antibody

(0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.
Goat Anti-ABCB5 Polyclonal Antibody – Cat# MBS421569

Flow cytometric (FC) of anti-ABCB5 antibody

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton.
Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml).
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Goat Anti-ABCB5 Polyclonal Antibody, Flow cytometric (FC) – Cat# MBS421569

Other Notes

Small volumes of anti-ABCB5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ABCB5. It may not necessarily be applicable to this product.

NCBI GI #
148612844
NCBI GeneID
340273
NCBI Accession #
NP_848654.3
NCBI Related Accession #
NP_001157413.1
UniProt Secondary Accession #
Q2M3I5; Q5I5Q7; Q5I5Q8; Q6KG50; A4D131; A7BKA4; B5MD19; B7WPL1; F8QQP8; F8QQP9; J3KQ04
UniProt Related Accession #
Q2M3G0
GenBank Nucleotide #
NM_178559.5
NCBI Official Full Name
ATP-binding cassette sub-family B member 5 isoform 2
NCBI Official Synonym Full Names
ATP binding cassette subfamily B member 5
NCBI Official Symbol
ABCB5
NCBI Official Synonym Symbols
ABCB5beta; EST422562; ABCB5alpha
UniProt Protein Name
ATP-binding cassette sub-family B member 5
UniProt Synonym Protein Names
ABCB5 P-gp; P-glycoprotein ABCB5
Protein Family
ABC transporter B family
UniProt Gene Name
ABCB5
UniProt Entry Name
ABCB5_HUMAN
NCBI Protein Information
ATP-binding cassette sub-family B member 5

NCBI Summary for ABCB5

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]
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UniProt Comments for ABCB5

ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ABC family; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: ATP binding; ATPase activity, coupled to transmembrane movement of substances; efflux transmembrane transporter activity; xenobiotic-transporting ATPase activity

Biological Process: antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; cell differentiation; compound eye corneal lens development; metabolic process; multidrug transport; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; regulation of membrane potential; transmembrane transport; xenobiotic transport
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Research Articles on ABCB5

1. This is the first study showing the relationship between miR-340-5p and expression of ABCB5 in melanoma cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ABCB5 antibody

 Reference Product  PubMed Publications
 SOCS1 antibody  >1 publications with ABCB5 and SOCS1

Pathways associated with anti-ABCB5 antibody

 Products by Pathway  Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 ABC Transporters Pathway antibodies  ABC Transporters Pathway Diagram
 ABC-family Proteins Mediated Transport Pathway antibodies  ABC-family Proteins Mediated Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-ABCB5 antibody

 Disease Name  Pubmed Publications
 Melanoma Antibodies  >65 publications with ABCB5 and Melanoma
 Disease Models, Animal Antibodies  >4 publications with ABCB5 and Disease Models, Animal
 Liver Diseases Antibodies  >3 publications with ABCB5 and Liver Diseases
 Colorectal Neoplasms Antibodies  >3 publications with ABCB5 and Colorectal Neoplasms
 Inflammation Antibodies  >2 publications with ABCB5 and Inflammation
 Necrosis Antibodies  >2 publications with ABCB5 and Necrosis
 Hyperplasia Antibodies  >2 publications with ABCB5 and Hyperplasia
 Neurotoxicity Syndromes Antibodies  >1 publications with ABCB5 and Neurotoxicity Syndromes
 Liver Failure Antibodies  >1 publications with ABCB5 and Liver Failure
 Hypertension Antibodies  >1 publications with ABCB5 and Hypertension

Organs/Tissues associated with anti-ABCB5 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >30 publications with ABCB5 and Skin
 Testis Antibodies  >3 publications with ABCB5 and Testis
 Brain Antibodies  >3 publications with ABCB5 and Brain
 Intestine Antibodies  >2 publications with ABCB5 and Intestine
 Kidney Antibodies  >1 publications with ABCB5 and Kidney
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