• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NEBL Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421779

MBS421779 is an antibody targeting NEBL. Also known as Goat anti-NEBL/nebulette Antibody. Reactive in Expected from sequence similarity: Human, Mouse, Dog. Host: Goat. More details

$305 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEBL/nebulette, Polyclonal Antibody
Full Product Name
Goat anti-NEBL/nebulette Antibody
Product Gene Name
Product Synonym Names
NEBL; nebulette; FLJ53769; LNEBL; MGC119746; MGC119747; bA56H7.1; LIM-nebulette; NEBL, actin-binding Z-disc protein; OTTHUMP00000019268; actin-binding Z-disk protein; NEBL antibody; nebulette antibody; FLJ53769 antibody; LNEBL antibody; MGC119746 antibody; MGC119747 antibody; bA56H7.1 antibody; LIM-nebulette antibody; NEBL; actin-binding Z-disc protein antibody; OTTHUMP00000019268 antibody; actin-binding Z-disk protein antibody; Nebulette; NEBL / nebulette
Immunogen Sequence
ELQRLKRTQE
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human, Mouse, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
31,196 Da
OMIM
605491
Immunogen
Peptide with sequence C-ELQRLKRTQE, from the internal region of the protein sequence according to NP_998734.1; NP_001166955.1.
Epitope
Internal region
Note
This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1; NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NEBL antibody
Peptide ELISA (EIA)
Application Notes for anti-NEBL antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Preliminary experiments gave an approx 30kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1ug/ml antibody staining. This protein has a calculated MW of 31.2kDa according to NP_998734.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide.

Other Notes

Small volumes of anti-NEBL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEBL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nebulette isoform 2
NCBI Official Synonym Full Names
nebulette
NCBI Official Symbol
NEBL
NCBI Official Synonym Symbols
LASP2; LNEBL
UniProt Protein Name
Nebulette
UniProt Synonym Protein Names
Actin-binding Z-disk protein
UniProt Gene Name
NEBL
UniProt Synonym Gene Names
LNEBL
UniProt Entry Name
NEBL_HUMAN
NCBI Protein Information
nebulette

NCBI Summary for NEBL

This gene encodes a nebulin like protein that is abundantly expressed in cardiac muscle. The encoded protein binds actin and interacts with thin filaments and Z-line associated proteins in striated muscle. This protein may be involved in cardiac myofibril assembly. A shorter isoform of this protein termed LIM nebulette is expressed in non-muscle cells and may function as a component of focal adhesion complexes. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
Show more

UniProt Comments for NEBL

NEBL: Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 10p12

Cellular Component: I band; stress fiber; Z disc

Molecular Function: actin filament binding; cytoskeletal protein binding; filamin binding; protein binding; structural constituent of muscle; tropomyosin binding
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000