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WIZ Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421804

MBS421804 is an antibody targeting WIZ. Also known as Goat anti-WIZ Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
WIZ, Polyclonal Antibody
Full Product Name
Goat anti-WIZ Antibody
Product Gene Name
Product Synonym Names
WIZ; widely interspaced zinc finger motifs; ZNF803; WIZ zinc finger; widely-interspaced zinc finger motifs; WIZ antibody; widely interspaced zinc finger motifs antibody; ZNF803 antibody; WIZ zinc finger antibody; widely-interspaced zinc finger motifs antibody
Immunogen Sequence
RSYIQGGRPF TKKFR
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Antibody/Peptide Pairs
WIZ peptide (MBS425525) is used for blocking the activity of WIZ antibody (MBS421804)
Immunogen
Peptide with sequence C-RSYIQGGRPFTKKFR, from the internal region of the protein sequence according to NP_067064.2.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-WIZ antibody
Peptide ELISA (EIA), Western Blot (WB), Flow Cytometry (FC)
Application Notes for anti-WIZ antibody
Peptide ELISA: antibody detection limit dilution 1:2000.

Western blot: Approx 75kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 85.4kDa according to NP_067064.2). An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Western Blot (WB) of anti-WIZ antibody

(0.3ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-WIZ Polyclonal Antibody – Cat# MBS421804

Western Blot (WB) of anti-WIZ antibody

(MBS421804) (1µg/ml) staining of Human Cerebellum lysate (A) + Blocking peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.
Goat Anti-WIZ Polyclonal Antibody, Western Blot (WB) – Cat# MBS421804

Western Blot (WB) of anti-WIZ antibody

(MBS421804) Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Goat Anti-WIZ Polyclonal Antibody, Western Blot (WB) – Cat# MBS421804

Other Notes

Small volumes of anti-WIZ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WIZ. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein Wiz
NCBI Official Synonym Full Names
widely interspaced zinc finger motifs
NCBI Official Symbol
WIZ
NCBI Official Synonym Symbols
ZNF803
UniProt Protein Name
Protein Wiz
UniProt Synonym Protein Names
Widely-interspaced zinc finger-containing protein; Zinc finger protein 803
Protein Family
UniProt Gene Name
WIZ
UniProt Synonym Gene Names
ZNF803
UniProt Entry Name
WIZ_HUMAN
NCBI Protein Information
protein Wiz

UniProt Comments for WIZ

WIZ: May link EHMT1 and EHMT2 histone methyltransferases to the CTBP corepressor machinery. May be involved in EHMT1-EHMT2 heterodimer formation and stabilization. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: nucleus

Molecular Function: metal ion binding; protein binding

Biological Process: protein stabilization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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