Goat Anti-APOBEC1 Polyclonal Antibody – Cat# MBS421886

(1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

APOBEC1 Antibody – Goat Polyclonal

Name: APOBEC1 Antibody; Polyclonal; Goat; Mouse/Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS421886
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421886

MBS421886 is an antibody targeting APOBEC1. Also known as Goat anti-APOBEC1 Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Human, Mouse. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

APOBEC1, Polyclonal Antibody

Full Product Name

Goat anti-APOBEC1 Antibody

Product Gene Name

anti-APOBEC1 antibody

Product Synonym Names

APOBEC1; apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1; APOBEC-1; BEDP; CDAR1; HEPR; apolipoprotein B mRNA editing enzyme; apolipoprotein B mRNA editing enzyme complex-1; APOBEC1 antibody; apolipoprotein B mRNA editing enzyme; catalytic polypeptide 1 antibody; APOBEC-1 antibody; BEDP antibody; CDAR1 antibody; HEPR antibody; apolipoprotein B mRNA editing enzyme antibody; apolipoprotein B mRNA editing enzyme complex-1 antibody

Immunogen Sequence

DVFYDPRELR KEAC

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Mouse; Expected from sequence similarity: Human, Mouse

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

APOBEC1 peptide (MBS425185) is used for blocking the activity of APOBEC1 antibody (MBS421886)

Molecular Weight

28,192 Da

OMIM

600130

Immunogen

Peptide with sequence DVFYDPRELRKEAC, from the internal region (near N Terminus) of the protein sequence according to NP_001635.2.

Epitope

Internal region (near N Terminus)

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-APOBEC1 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-APOBEC1 antibody

Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx.28kDa band observed in Mouse Spleen lysates (calculated MW of 28.2kDa according to Human NP_001635.2 and 27.5kDa acccording to Mouse NP_112436.1). Recommended concentration: 1-3ug/ml.

Other Notes

Small volumes of anti-APOBEC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for APOBEC1. It may not necessarily be applicable to this product.

NCBI GI #

61743957

NCBI GeneID

339

NCBI Accession #

NP_001635.2

UniProt Secondary Accession #

Q9UE64; Q9UM71

UniProt Related Accession #

P41238

GenBank Nucleotide #

NM_001644.4

NCBI Official Full Name

C->U-editing enzyme APOBEC-1 isoform a

NCBI Official Synonym Full Names

apolipoprotein B mRNA editing enzyme catalytic subunit 1

NCBI Official Symbol

APOBEC1

NCBI Official Synonym Symbols

BEDP; HEPR; CDAR1; APOBEC-1

UniProt Protein Name

C->U-editing enzyme APOBEC-1

UniProt Synonym Protein Names

Apolipoprotein B mRNA-editing enzyme 1; HEPR; mRNA(cytosine(6666)) deaminase 1

Protein Family

C->U-editing enzyme

UniProt Gene Name

APOBEC1

UniProt Entry Name

ABEC1_HUMAN

NCBI Protein Information

C->U-editing enzyme APOBEC-1

NCBI Summary for APOBEC1

This gene encodes a member of the cytidine deaminase enzyme family. The encoded protein forms a multiple-protein editing holoenzyme with APOBEC1 complementation factor (ACF) and APOBEC1 stimulating protein (ASP). This holoenzyme is involved in the editing of C-to-U nucleotide bases in apolipoprotein B and neurofibromatosis-1 mRNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2015]

UniProt Comments for APOBEC1

APOBEC1: apolipoprotein B mRNA editing enzyme. Responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. Expressed exclusively in the small intestine.

Protein type: Cell cycle regulation; RNA processing; Hydrolase; RNA-binding; EC 3.5.4.-

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: cytoplasm; nucleoplasm

Molecular Function: AU-rich element binding; cytidine deaminase activity; cytosine deaminase activity; enzyme activator activity; mRNA binding; protein binding; protein domain specific binding; ribonucleoprotein binding; RNA binding; zinc ion binding

Biological Process: cellular response to insulin stimulus; cytidine deamination; cytidine to uridine editing; defense response to virus; lipid metabolic process; lipoprotein biosynthetic process; lipoprotein transport; mRNA modification; mRNA processing; mRNA stabilization; positive regulation of catalytic activity; regulation of cell proliferation; response to calcium ion; response to drug; response to ethanol; response to gamma radiation; response to osmotic stress; response to zinc ion; RNA processing

Research Articles on APOBEC1

1. Results show that expression of APOBEC1 induces a mutator phenotype in 2 different cellular models.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-APOBEC1 antibody

Products by Pathway	Pathway Diagram
Formation Of The Editosome Pathway antibodies	Formation Of The Editosome Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
MRNA Editing Pathway antibodies	MRNA Editing Pathway Diagram
MRNA Editing: C To U Conversion Pathway antibodies	MRNA Editing: C To U Conversion Pathway Diagram

Diseases associated with anti-APOBEC1 antibody

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>4 publications with APOBEC1 and Heart Diseases
Nervous System Diseases Antibodies	>3 publications with APOBEC1 and Nervous System Diseases
Inflammation Antibodies	>3 publications with APOBEC1 and Inflammation
Neoplasms, Experimental Antibodies	>2 publications with APOBEC1 and Neoplasms, Experimental
Liver Diseases Antibodies	>2 publications with APOBEC1 and Liver Diseases
Adenocarcinoma Antibodies	>2 publications with APOBEC1 and Adenocarcinoma
Carcinoma, Hepatocellular Antibodies	>2 publications with APOBEC1 and Carcinoma, Hepatocellular
Testicular Diseases Antibodies	>2 publications with APOBEC1 and Testicular Diseases
Fibrosis Antibodies	>1 publications with APOBEC1 and Fibrosis
Cell Transformation, Neoplastic Antibodies	>1 publications with APOBEC1 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-APOBEC1 antibody

Organ/Tissue Name	Pubmed Publications
Intestine Antibodies	>12 publications with APOBEC1 and Intestine