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Calponin 2/CNN2 (CNN2) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421943

MBS421943 is an antibody targeting CNN2. Also known as Goat anti-Calponin 2/CNN2 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Calponin 2/CNN2, Polyclonal Antibody
Full Product Name
Goat anti-Calponin 2/CNN2 Antibody
Product Gene Name
Product Synonym Names
CNN2; calponin 2; calponin H2, smooth muscle; neutral calponin; CNN2 antibody; calponin 2 antibody; calponin H2; smooth muscle antibody; neutral calponin antibody; Calponin 2; Calponin 2 / CNN2
Immunogen Sequence
DPGEVPEYPP YYQEE
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
Molecular Weight
29,500 Da
OMIM
602373
Immunogen
Peptide with sequence C-DPGEVPEYPPYYQEE, from the internal region (near C Terminus) of the protein sequence according to NP_004359.1; NP_958434.1.
Epitope
Internal region (near C Terminus)
Note
This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with CCN3.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-CNN2 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-CNN2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 35kDa band observed in lysates of cell line HepG2 (calculated MW of 33.7kDa according to NP_004359.1). Recommended concentration: 0. 03-0.1ug/ml.

Western Blot (WB) of anti-CNN2 antibody

(0. 03ug/ml) staining of HepG2 lysates (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-CNN2 Polyclonal Antibody – Cat# MBS421943

Other Notes

Small volumes of anti-CNN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CNN2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
calponin-2 isoform a
NCBI Official Synonym Full Names
calponin 2
NCBI Official Symbol
CNN2
UniProt Protein Name
Calponin-2
UniProt Synonym Protein Names
Calponin H2, smooth muscle; Neutral calponin
Protein Family
UniProt Gene Name
CNN2
UniProt Entry Name
CNN2_HUMAN
NCBI Protein Information
calponin-2

NCBI Summary for CNN2

The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Several pseudogenes of this gene have been identified, and are present on chromosomes 1, 2, 3, 6, 9, 11, 13, 15, 16, 21 and 22. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]
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UniProt Comments for CNN2

calponin 2: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoskeleton; focal adhesion; intercellular junction; membrane; stress fiber

Molecular Function: actin binding; calmodulin binding

Biological Process: actomyosin structure organization and biogenesis; cytoskeleton organization and biogenesis; regulation of actin filament-based process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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