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ATG16L1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS421951

MBS421951 is an antibody targeting ATG16L1. Also known as Goat anti-ATG16L1 Antibody. Reactive in Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ATG16L1, Polyclonal Antibody
Full Product Name
Goat anti-ATG16L1 Antibody
Product Gene Name
Product Synonym Names
ATG16; autophagy related 16-like 1 (S. cerevisiae); hCG_1817841; APG16L; ATG16L; FLJ00045; FLJ10035; FLJ10828; FLJ22677; WDR30; APG16 autophagy 16-like; APG16L beta; ATG16 autophagy related 16-like protein 1; WD repeat domain 30; ATG16 antibody; autophagy related 16-like 1 (S. cerevisiae) antibody; hCG_1817841 antibody; APG16L antibody; ATG16L antibody; FLJ00045 antibody; FLJ10035 antibody; FLJ10828 antibody; FLJ22677 antibody; WDR30 antibody; APG16 autophagy 16-like antibody; APG16L beta antibody; ATG16 autophagy related 16-like protein 1 antibody; WD repeat domain 30 antibody
Immunogen Sequence
KVEKVLSKQH SSSIN
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
ATG16L1 peptide (MBS426429) is used for blocking the activity of ATG16L1 antibody (MBS421951)
Molecular Weight
49,486 Da
OMIM
610767
Immunogen
Peptide with sequence C-KVEKVLSKQHSSSIN, from the internal region (near the C Terminus) of the protein sequence according to NP_110430.5; NP_060444.3.
Epitope
Internal region (near the C Terminus)
Note
This antibody is expected to recognise both reported isoforms (NP_110430.5 and NP_060444.3).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-ATG16L1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-ATG16L1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 65kDa band observed in Mouse Brain lysates (calculated MW of 68.1kDa according to Mouse NP_084122.2). Recommended concentration: 0. 01-0.1ug/ml.

Western Blot (WB) of anti-ATG16L1 antibody

(0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-ATG16L1 Polyclonal Antibody – Cat# MBS421951

Other Notes

Small volumes of anti-ATG16L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG16L1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
autophagy-related protein 16-1 isoform 1
NCBI Official Synonym Full Names
autophagy related 16 like 1
NCBI Official Symbol
ATG16L1
NCBI Official Synonym Symbols
IBD10; WDR30; APG16L; ATG16A; ATG16L
UniProt Protein Name
Autophagy-related protein 16-1
UniProt Synonym Protein Names
APG16-like 1
UniProt Gene Name
ATG16L1
UniProt Synonym Gene Names
APG16L
UniProt Entry Name
A16L1_HUMAN
NCBI Protein Information
autophagy-related protein 16-1

NCBI Summary for ATG16L1

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]
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UniProt Comments for ATG16L1

ATG16L1: Plays an essential role in autophagy. Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Belongs to the WD repeat ATG16 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, peripheral; Autophagy

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: autophagic vacuole; axoneme; cytosol; pre-autophagosomal structure membrane

Molecular Function: identical protein binding; protein binding; small conjugating protein ligase activity

Biological Process: autophagic vacuole formation; macroautophagy; protein homooligomerization; protein transport

Disease: Inflammatory Bowel Disease 10
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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